Best2 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_168162 · chr3L

115 Specific

FWD 5' GTTGAGAGCACAACGCCAAC 3'

REV 5' CATTAGGACCGAGCTGGTGG 3'

Fwd Tm 61.8°C

Rev Tm 62.2°C

GC 55%

Product 145 bp

Spans 2 introns

NM_168162 · chr3L

115 Specific

FWD 5' GTTGAGAGCACAACGCCAAC 3'

REV 5' CCATTAGGACCGAGCTGGTG 3'

Fwd Tm 61.8°C

Rev Tm 62.2°C

GC 55%

Product 146 bp

Spans 2 introns

NM_168162 · chr3L

114 Specific

FWD 5' TTGAGAGCACAACGCCAACT 3'

REV 5' CATTAGGACCGAGCTGGTGG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 50%

Product 144 bp

Spans 2 introns

NM_168162 · chr3L

114 Specific

FWD 5' TTGAGAGCACAACGCCAACT 3'

REV 5' CCATTAGGACCGAGCTGGTG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 50%

Product 145 bp

Spans 2 introns

NM_168162 · chr3L

114 Specific

FWD 5' TGAGAGCACAACGCCAACTT 3'

REV 5' CATTAGGACCGAGCTGGTGG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 50%

Product 143 bp

Spans 2 introns

NM_168162 · chr3L

114 Specific

FWD 5' TGAGAGCACAACGCCAACTT 3'

REV 5' CCATTAGGACCGAGCTGGTG 3'

Fwd Tm 62.0°C

Rev Tm 62.2°C

GC 50%

Product 144 bp

Spans 2 introns

NM_168162 · chr3L

113 Specific

FWD 5' TGAGAGCACAACGCCAACTT 3'

REV 5' GTGGTGCCATTAGGACCGAG 3'

Fwd Tm 62.0°C

Rev Tm 62.4°C

GC 50%

Product 150 bp

Spans 2 introns

NM_168162 · chr3L

113 Specific

FWD 5' GTTGAGAGCACAACGCCAAC 3'

REV 5' GTGCCATTAGGACCGAGCTG 3'

Fwd Tm 61.8°C

Rev Tm 62.7°C

GC 55%

Product 149 bp

Spans 2 introns

NM_168162 · chr3L

112 Specific

FWD 5' TTGAGAGCACAACGCCAACT 3'

REV 5' GTGCCATTAGGACCGAGCTG 3'

Fwd Tm 62.0°C

Rev Tm 62.7°C

GC 50%

Product 148 bp

Spans 2 introns

NM_168162 · chr3L

112 Specific

FWD 5' TGAGAGCACAACGCCAACTT 3'

REV 5' GTGCCATTAGGACCGAGCTG 3'

Fwd Tm 62.0°C

Rev Tm 62.7°C

GC 50%

Product 147 bp

Spans 2 introns