BigH1 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_142126 · chr3R

109 Specific

FWD 5' GAAAGAGGCCAAGGCGAAGA 3'

REV 5' GCTGGCGGATTATCCTTGGT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 132 bp

Spans 1 intron

NM_142126 · chr3R

109 Specific

FWD 5' GAAAGAGGCCAAGGCGAAGA 3'

REV 5' TGCTGGCGGATTATCCTTGG 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 133 bp

Spans 1 intron

NM_142126 · chr3R

109 Specific

FWD 5' TCCAGTAAACCAACCGAGCG 3'

REV 5' CGCTGTCTGGGATGTTTCCA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 141 bp

Spans 1 intron

NM_142126 · chr3R

109 Specific

FWD 5' CAGTAAACCAACCGAGCGGA 3'

REV 5' CGCTGTCTGGGATGTTTCCA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 139 bp

Spans 1 intron

NM_142126 · chr3R

109 Specific

FWD 5' TCCAGTAAACCAACCGAGCG 3'

REV 5' TCGCTGTCTGGGATGTTTCC 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 142 bp

Spans 1 intron

NM_142126 · chr3R

109 Specific

FWD 5' CAGTAAACCAACCGAGCGGA 3'

REV 5' TCGCTGTCTGGGATGTTTCC 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 140 bp

Spans 1 intron

NM_142126 · chr3R

109 Specific

FWD 5' AGAAAGAGGCCAAGGCGAAG 3'

REV 5' GCTGGCGGATTATCCTTGGT 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 133 bp

Spans 1 intron

NM_142126 · chr3R

109 Specific

FWD 5' AGAAAGAGGCCAAGGCGAAG 3'

REV 5' TGCTGGCGGATTATCCTTGG 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 134 bp

Spans 1 intron

NM_142126 · chr3R

109 Specific

FWD 5' AGAAAGAGGCCAAGGCGAAG 3'

REV 5' CTGCTTTAGATGCTGGCGGA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 144 bp

Spans 1 intron

NM_142126 · chr3R

109 Specific

FWD 5' GAGGCCAAGGCGAAGATGAA 3'

REV 5' CTGCTTTAGATGCTGGCGGA 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 139 bp

Spans 1 intron