Blos1 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_166060 · chr2R

109 Specific

FWD 5' ATCACAAGGAGCAGGCGAAG 3'

REV 5' ACGCCCACATTTAGGGTGTC 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 112 bp

Spans 1 intron

NM_166060 · chr2R

109 Specific

FWD 5' ATCACAAGGAGCAGGCGAAG 3'

REV 5' GCCCACATTTAGGGTGTCCA 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 110 bp

Spans 1 intron

NM_166060 · chr2R

108 Specific

FWD 5' AGGAGCATCACAAGGAGCAG 3'

REV 5' GCCCACATTTAGGGTGTCCA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 116 bp

Spans 1 intron

NM_166060 · chr2R

108 Specific

FWD 5' GAACAGGAGGTGCGTCGAAA 3'

REV 5' GTGCAGTTGCTTTGCCTCTG 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 138 bp

Spans 1 intron

NM_166060 · chr2R

108 Specific

FWD 5' CATCACAAGGAGCAGGCGAA 3'

REV 5' TGAGCCACGCCCACATTTAG 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 119 bp

Spans 1 intron

NM_166060 · chr2R

108 Specific

FWD 5' CATCACAAGGAGCAGGCGAA 3'

REV 5' CTGAGCCACGCCCACATTTA 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 120 bp

Spans 1 intron

NM_166060 · chr2R

108 Specific

FWD 5' AACAGGAGGTGCGTCGAAAG 3'

REV 5' GTGCAGTTGCTTTGCCTCTG 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 137 bp

Spans 1 intron

NM_166060 · chr2R

108 Specific

FWD 5' CATCACAAGGAGCAGGCGAA 3'

REV 5' ACGCCCACATTTAGGGTGTC 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 113 bp

Spans 1 intron

NM_166060 · chr2R

108 Specific

FWD 5' CATCACAAGGAGCAGGCGAA 3'

REV 5' CACGCCCACATTTAGGGTGT 3'

Fwd Tm 62.4°C

Rev Tm 62.5°C

GC 55%

Product 114 bp

Spans 1 intron

NM_166060 · chr2R

108 Specific

FWD 5' AAACAGGAACAGGAGGTGCG 3'

REV 5' TGAGCCACGCCCACATTTAG 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 95 bp

Spans 1 intron