Buffy 10 primer pairs found

Drosophila

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NM_078978 · chr2R

109 Specific

FWD 5' TAGCATACTGGGCTCCACCT 3'

REV 5' CCGGATTCCGGCAAATCTGA 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 134 bp

Spans 1 intron

NM_078978 · chr2R

109 Specific

FWD 5' CACAGATTCCAGCGCAGGAT 3'

REV 5' AGGTGGAGCCCAGTATGCTA 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 138 bp

Spans 1 intron

NM_078978 · chr2R

109 Specific

FWD 5' TTTCTCAGGGTCGTTGCCTG 3'

REV 5' AGGTGGAGCCCAGTATGCTA 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 114 bp

Spans 1 intron

NM_078978 · chr2R

109 Specific

FWD 5' CGCGCTTCTCAAGTTTCAGC 3'

REV 5' ATTCGTGGGATACGAGGTGC 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 109 bp

Spans 1 intron

NM_078978 · chr2R

108 Specific

FWD 5' GATGTGTTTCCAGCGGTCCA 3'

REV 5' CCGGATTCCGGCAAATCTGA 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 97 bp

Spans 1 intron

NM_078978 · chr2R

108 Specific

FWD 5' ATACTGGGCTCCACCTCCAT 3'

REV 5' TATGGAACTCACCACCCGGA 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 145 bp

Spans 1 intron

NM_078978 · chr2R

108 Specific

FWD 5' GATGTGTTTCCAGCGGTCCA 3'

REV 5' TATGGAACTCACCACCCGGA 3'

Fwd Tm 62.4°C

Rev Tm 62.4°C

GC 55%

Product 112 bp

Spans 1 intron

NM_078978 · chr2R

108 Specific

FWD 5' ATACTGGGCTCCACCTCCAT 3'

REV 5' CCGGATTCCGGCAAATCTGA 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 130 bp

Spans 1 intron

NM_078978 · chr2R

108 Specific

FWD 5' CACAGATTCCAGCGCAGGAT 3'

REV 5' ATGGAGGTGGAGCCCAGTAT 3'

Fwd Tm 62.2°C

Rev Tm 62.4°C

GC 55%

Product 142 bp

Spans 1 intron

NM_078978 · chr2R

108 Specific

FWD 5' CGCCAAGGACATCCGGAATA 3'

REV 5' TTCAGGCTGTTGGTAGTGGG 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 143 bp

Spans 1 intron