Bug22 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_135558 · chr2L

109 Specific

FWD 5' TAACATGTCCGGCAGATCCG 3'

REV 5' TACTGGCCCTAAACCTTCGC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 144 bp

Spans 1 intron

NM_135558 · chr2L

108 Specific

FWD 5' GCAGATCCGAAGAAGACGCT 3'

REV 5' TACTGGCCCTAAACCTTCGC 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 133 bp

Spans 1 intron

NM_135558 · chr2L

108 Specific

FWD 5' CTGCCCTTCCTGGTGATGAT 3'

REV 5' TGACACGAGTTGTCGACTGG 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 127 bp

Spans 1 intron

NM_135558 · chr2L

108 Specific

FWD 5' CTGCCCTTCCTGGTGATGAT 3'

REV 5' GACACGAGTTGTCGACTGGT 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 126 bp

Spans 1 intron

NM_135558 · chr2L

108 Specific

FWD 5' CTGCCCTTCCTGGTGATGAT 3'

REV 5' TACTGGCCCTAAACCTTCGC 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 103 bp

Spans 1 intron

NM_135558 · chr2L

108 Specific

FWD 5' AGAAGACGCTGGGCATCAAG 3'

REV 5' CCGCACGTTCTTGTCATCGA 3'

Fwd Tm 62.2°C

Rev Tm 62.4°C

GC 55%

Product 104 bp

Spans 1 intron

NM_135558 · chr2L

108 Specific

FWD 5' TGCCCTTCCTGGTGATGATC 3'

REV 5' TGACACGAGTTGTCGACTGG 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 126 bp

Spans 1 intron

NM_135558 · chr2L

108 Specific

FWD 5' GCCCTTCCTGGTGATGATCA 3'

REV 5' TGACACGAGTTGTCGACTGG 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 125 bp

Spans 1 intron

NM_135558 · chr2L

108 Specific

FWD 5' TGCCCTTCCTGGTGATGATC 3'

REV 5' GACACGAGTTGTCGACTGGT 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 125 bp

Spans 1 intron

NM_135558 · chr2L

108 Specific

FWD 5' GCCCTTCCTGGTGATGATCA 3'

REV 5' GACACGAGTTGTCGACTGGT 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 124 bp

Spans 1 intron