C15 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_057525 · chr3R

109 Specific

FWD 5' AAGACGTGGTTCCAGAACCG 3'

REV 5' AATCCCTTGCTGATGGCCTC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 125 bp

Spans 1 intron

NM_057525 · chr3R

109 Specific

FWD 5' AAGACGTGGTTCCAGAACCG 3'

REV 5' GAATCCCTTGCTGATGGCCT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 126 bp

Spans 1 intron

NM_057525 · chr3R

108 Specific

FWD 5' ACATCAGGCGGTCAAGGATC 3'

REV 5' TGGAAGCGCTTCTCCAACTC 3'

Fwd Tm 61.7°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 1 intron

NM_057525 · chr3R

108 Specific

FWD 5' AAGACGTGGTTCCAGAACCG 3'

REV 5' ACAGCATCAGTCTGTTCGCC 3'

Fwd Tm 62.0°C

Rev Tm 62.3°C

GC 55%

Product 94 bp

Spans 1 intron

NM_057525 · chr3R

108 Specific

FWD 5' TGCTACGACGAGTCCGAAAC 3'

REV 5' AGGCGTGATATGCTGAAGGG 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 98 bp

Spans 1 intron

NM_057525 · chr3R

108 Specific

FWD 5' TGCTACGACGAGTCCGAAAC 3'

REV 5' TGTTGTGGTGGTGGTGACTC 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 139 bp

Spans 1 intron

NM_057525 · chr3R

108 Specific

FWD 5' GACGAGTCCGAAACGCCATT 3'

REV 5' GCGTGATATGCTGAAGGGCA 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 90 bp

Spans 1 intron

NM_057525 · chr3R

108 Specific

FWD 5' CAGGTGAAGACGTGGTTCCA 3'

REV 5' AATCCCTTGCTGATGGCCTC 3'

Fwd Tm 61.7°C

Rev Tm 62.1°C

GC 55%

Product 131 bp

Spans 1 intron

NM_057525 · chr3R

108 Specific

FWD 5' CAGGTGAAGACGTGGTTCCA 3'

REV 5' GAATCCCTTGCTGATGGCCT 3'

Fwd Tm 61.7°C

Rev Tm 62.1°C

GC 55%

Product 132 bp

Spans 1 intron

NM_057525 · chr3R

108 Specific

FWD 5' CGAGTCCGAAACGCCATTGA 3'

REV 5' GCTTGGATAGCAGGCGTGAT 3'

Fwd Tm 62.5°C

Rev Tm 62.3°C

GC 55%

Product 101 bp

Spans 1 intron