CG10274 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_001104055 · chr3L

109 Specific

FWD 5' GATCCACGAGATGACGCACA 3'

REV 5' CAATAGCACGAAGCACACGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 128 bp

Spans 1 intron

NM_001104055 · chr3L

109 Specific

FWD 5' CGCTGTGAGTGCAAGGTTTG 3'

REV 5' CAATAGCACGAAGCACACGC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 97 bp

Spans 1 intron

NM_001104055 · chr3L

109 Specific

FWD 5' GCCTGCAAGATCCACGAGAT 3'

REV 5' CAATAGCACGAAGCACACGC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 136 bp

Spans 1 intron

NM_001104055 · chr3L

109 Specific

FWD 5' GCACATGAAGCACCACAACG 3'

REV 5' CGTGGTCGATGTGAAGTCGT 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 104 bp

Spans 1 intron

NM_001104055 · chr3L

109 Specific

FWD 5' CCCTTCCAGTGCAAAGTGGA 3'

REV 5' AGCCATCGACATTGCAGGAG 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 121 bp

Spans 1 intron

NM_001104055 · chr3L

109 Specific

FWD 5' CATCCGCTACGAAGAGCACA 3'

REV 5' CGTGGTCGATGTGAAGTCGT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001104055 · chr3L

109 Specific

FWD 5' TCCGCTACGAAGAGCACATG 3'

REV 5' CGTGGTCGATGTGAAGTCGT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 117 bp

Spans 1 intron

NM_001104055 · chr3L

109 Specific

FWD 5' CCGCTACGAAGAGCACATGA 3'

REV 5' CGTGGTCGATGTGAAGTCGT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 116 bp

Spans 1 intron

NM_001104055 · chr3L

109 Specific

FWD 5' GCACATGAAGCACCACAACG 3'

REV 5' AGCCATCGACATTGCAGGAG 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001104055 · chr3L

109 Specific

FWD 5' CGCTGTGAGTGCAAGGTTTG 3'

REV 5' AACTGTTCACCAGGCGTCTC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 1 intron