CG1136 10 primer pairs found

Drosophila

Show all (including non-specific)

NM_139596 · chr3L

110 Specific

FWD 5' AGACCATCTACGTGGGCCTA 3'

REV 5' CCAGACTGTTGGCCGAAGAT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 123 bp

Spans 1 intron

NM_139596 · chr3L

109 Specific

FWD 5' GGATGGCACAGTCATCGGAA 3'

REV 5' TAGGCCCACGTAGATGGTCT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 124 bp

Spans 1 intron

NM_139596 · chr3L

109 Specific

FWD 5' GTGTGTACGGAGCCGTGAAA 3'

REV 5' AGTAACAACTGCTGCCAGGG 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 139 bp

Spans 1 intron

NM_139596 · chr3L

109 Specific

FWD 5' TGGCACAGTCATCGGAACTG 3'

REV 5' TAGGCCCACGTAGATGGTCT 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 121 bp

Spans 1 intron

NM_139596 · chr3L

109 Specific

FWD 5' GGCACAGTCATCGGAACTGA 3'

REV 5' TAGGCCCACGTAGATGGTCT 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 120 bp

Spans 1 intron

NM_139596 · chr3L

109 Specific

FWD 5' AGACCATCTACGTGGGCCTA 3'

REV 5' ATAGCTGCCCAGACTGTTGG 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 131 bp

Spans 1 intron

NM_139596 · chr3L

109 Specific

FWD 5' TCTTGTGCGTGTGTACGGAG 3'

REV 5' AGTAACAACTGCTGCCAGGG 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 147 bp

Spans 1 intron

NM_139596 · chr3L

108 Specific

FWD 5' CACCATGAAATCGTGGCTGC 3'

REV 5' TCCCTCGTCCGTCTGGATAT 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 103 bp

Spans 1 intron

NM_139596 · chr3L

108 Specific

FWD 5' TGCAGGATGGCACAGTCATC 3'

REV 5' TAGGCCCACGTAGATGGTCT 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 128 bp

Spans 1 intron

NM_139596 · chr3L

108 Specific

FWD 5' AGACCATCTACGTGGGCCTA 3'

REV 5' AGACTGTTGGCCGAAGATCC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 121 bp

Spans 1 intron