CG1146 40 primer pairs found

Drosophila

Show all (including non-specific)

NM_167951 · chr3L

100 1 gene · 2 transcripts

FWD 5' TTGAGCTCTCGATTCGCCAG 3'

REV 5' GCAATTCGATTTGCTTGGCG 3'

Fwd Tm 61.8°C

Rev Tm 60.5°C

GC 55%

Product 146 bp

Spans 1 intron

NM_167951 · chr3L

100 1 gene · 2 transcripts

FWD 5' GCTCTCGATTCGCCAGACAT 3'

REV 5' GCAATTCGATTTGCTTGGCG 3'

Fwd Tm 61.9°C

Rev Tm 60.5°C

GC 55%

Product 142 bp

Spans 1 intron

NM_167951 · chr3L

99 1 gene · 2 transcripts

FWD 5' TTGAGCTCTCGATTCGCCAG 3'

REV 5' GTAGTCCGTCATGCTCGCTT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 180 bp

Spans 1 intron

NM_167951 · chr3L

99 1 gene · 2 transcripts

FWD 5' GCTCTCGATTCGCCAGACAT 3'

REV 5' GTAGTCCGTCATGCTCGCTT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 176 bp

Spans 1 intron

NM_167951 · chr3L

99 1 gene · 2 transcripts

FWD 5' TTGAGCTCTCGATTCGCCAG 3'

REV 5' TTCCGTGTAGTCCGTCATGC 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 186 bp

Spans 1 intron

NM_167951 · chr3L

99 1 gene · 2 transcripts

FWD 5' GCTCTCGATTCGCCAGACAT 3'

REV 5' TTCCGTGTAGTCCGTCATGC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 182 bp

Spans 1 intron

NM_167951 · chr3L

99 1 gene · 2 transcripts

FWD 5' GCTCTCGATTCGCCAGACAT 3'

REV 5' AGGCGGTTTCCACGTATTCC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 198 bp

Spans 1 intron

NM_167951 · chr3L

98 1 gene · 2 transcripts

FWD 5' TTTGAGCTCTCGATTCGCCA 3'

REV 5' GCAATTCGATTTGCTTGGCG 3'

Fwd Tm 61.3°C

Rev Tm 60.5°C

GC 50%

Product 147 bp

Spans 1 intron

NM_167951 · chr3L

98 1 gene · 2 transcripts

FWD 5' TTGAGCTCTCGATTCGCCAG 3'

REV 5' CAATTCGATTTGCTTGGCGG 3'

Fwd Tm 61.8°C

Rev Tm 60.0°C

GC 55%

Product 145 bp

Spans 1 intron

NM_167951 · chr3L

98 1 gene · 2 transcripts

FWD 5' GCTCTCGATTCGCCAGACAT 3'

REV 5' CAATTCGATTTGCTTGGCGG 3'

Fwd Tm 61.9°C

Rev Tm 60.0°C

GC 55%

Product 141 bp

Spans 1 intron

NM_167950 · chr3L

95 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' TGTAGTCCGTCATGCTCGCT 3'

Fwd Tm 63.2°C

Rev Tm 63.0°C

GC 55%

Product 178 bp

Spans 1 intron

NM_167950 · chr3L

95 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' GTCCGTCATGCTCGCTTTGT 3'

Fwd Tm 63.2°C

Rev Tm 62.8°C

GC 55%

Product 174 bp

Spans 1 intron

NM_167950 · chr3L

95 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' TCCGTGTAGTCCGTCATGCT 3'

Fwd Tm 63.2°C

Rev Tm 62.8°C

GC 55%

Product 182 bp

Spans 1 intron

NM_167950 · chr3L

95 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' AGTCCGTCATGCTCGCTTTG 3'

Fwd Tm 63.2°C

Rev Tm 62.6°C

GC 55%

Product 175 bp

Spans 1 intron

NM_167950 · chr3L

95 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' GCGGTTTCCACGTATTCCGT 3'

Fwd Tm 63.2°C

Rev Tm 62.6°C

GC 55%

Product 197 bp

Spans 1 intron

NM_167950 · chr3L

94 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' AGGCGGTTTCCACGTATTCC 3'

Fwd Tm 63.2°C

Rev Tm 62.1°C

GC 55%

Product 199 bp

Spans 1 intron

NM_167950 · chr3L

94 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' GCAATTCGATTTGCTTGGCG 3'

Fwd Tm 63.2°C

Rev Tm 60.5°C

GC 55%

Product 143 bp

Spans 1 intron

NM_167950 · chr3L

93 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' GTAGTCCGTCATGCTCGCTT 3'

Fwd Tm 63.2°C

Rev Tm 61.8°C

GC 55%

Product 177 bp

Spans 1 intron

NM_167950 · chr3L

93 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' TTCCGTGTAGTCCGTCATGC 3'

Fwd Tm 63.2°C

Rev Tm 61.7°C

GC 55%

Product 183 bp

Spans 1 intron

NM_167950 · chr3L

92 Specific

FWD 5' CCCGCTGCGAGTTGCATAAA 3'

REV 5' GTGTAGTCCGTCATGCTCGC 3'

Fwd Tm 63.2°C

Rev Tm 62.7°C

GC 55%

Product 179 bp

Spans 1 intron

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