CG12009 20 primer pairs found

Drosophila

Show all (including non-specific)

NM_139542 · chr3L

109 1 gene · 2 transcripts

FWD 5' TTCCGCTGGAGCTTCATCTG 3'

REV 5' CTCGCACTCGATGGTCATGT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 93 bp

Spans 1 intron

NM_001259669 · chr3L

109 1 gene · 2 transcripts

FWD 5' TTCCGCTGGAGCTTCATCTG 3'

REV 5' CTCGCACTCGATGGTCATGT 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 93 bp

Spans 1 intron

NM_139542 · chr3L

109 1 gene · 2 transcripts

FWD 5' TTCCGCTGGAGCTTCATCTG 3'

REV 5' TCGCACTCGATGGTCATGTC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 92 bp

Spans 1 intron

NM_001259669 · chr3L

109 1 gene · 2 transcripts

FWD 5' TTCCGCTGGAGCTTCATCTG 3'

REV 5' TCGCACTCGATGGTCATGTC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 92 bp

Spans 1 intron

NM_139542 · chr3L

109 1 gene · 2 transcripts

FWD 5' TTCCGCTGGAGCTTCATCTG 3'

REV 5' TCTGGAGCTATCCTCGCACT 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 105 bp

Spans 1 intron

NM_001259669 · chr3L

109 1 gene · 2 transcripts

FWD 5' TTCCGCTGGAGCTTCATCTG 3'

REV 5' TCTGGAGCTATCCTCGCACT 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 105 bp

Spans 1 intron

NM_001259669 · chr3L

109 Specific

FWD 5' TGGGAGCAAGTCCAAGTTCC 3'

REV 5' ATTTCCACCTCAGCTGGGAC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 115 bp

Spans 1 intron

NM_001259669 · chr3L

109 Specific

FWD 5' TGGGAGCAAGTCCAAGTTCC 3'

REV 5' CATTTCCACCTCAGCTGGGA 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 116 bp

Spans 1 intron

NM_001259669 · chr3L

109 Specific

FWD 5' GGGAGCAAGTCCAAGTTCCA 3'

REV 5' ATTTCCACCTCAGCTGGGAC 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 114 bp

Spans 1 intron

NM_001259669 · chr3L

109 Specific

FWD 5' GGGAGCAAGTCCAAGTTCCA 3'

REV 5' CATTTCCACCTCAGCTGGGA 3'

Fwd Tm 61.9°C

Rev Tm 61.7°C

GC 55%

Product 115 bp

Spans 1 intron

NM_001259669 · chr3L

109 Specific

FWD 5' CGTGGGAGCAAGTCCAAGTT 3'

REV 5' TCGGTCTGTTCCGGCATTTC 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 132 bp

Spans 1 intron

NM_001259669 · chr3L

109 Specific

FWD 5' CGTGGGAGCAAGTCCAAGTT 3'

REV 5' TTCCGGCATTTCCACCTCAG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 124 bp

Spans 1 intron

NM_001259669 · chr3L

108 Specific

FWD 5' TGGGAGCAAGTCCAAGTTCC 3'

REV 5' TTCCGGCATTTCCACCTCAG 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 122 bp

Spans 1 intron

NM_139542 · chr3L

108 1 gene · 2 transcripts

FWD 5' GATGTGGAGAACGACTGCCA 3'

REV 5' CGCATGCAGGTGAACGATTC 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 137 bp

Spans 1 intron

NM_139542 · chr3L

108 1 gene · 2 transcripts

FWD 5' ATGTGGAGAACGACTGCCAG 3'

REV 5' CGCATGCAGGTGAACGATTC 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 136 bp

Spans 1 intron

NM_139542 · chr3L

108 1 gene · 2 transcripts

FWD 5' TTCCGCTGGAGCTTCATCTG 3'

REV 5' GCACTCGATGGTCATGTCCT 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 90 bp

Spans 1 intron

NM_139542 · chr3L

108 1 gene · 2 transcripts

FWD 5' GTGGAGAACGACTGCCAGAT 3'

REV 5' CGCATGCAGGTGAACGATTC 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 134 bp

Spans 1 intron

NM_139542 · chr3L

108 1 gene · 2 transcripts

FWD 5' GGAATCGTTCACCTGCATGC 3'

REV 5' GCTTACTTTCCTCCTCCGCA 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 113 bp

Spans 1 intron

NM_139542 · chr3L

108 1 gene · 2 transcripts

FWD 5' GGAATCGTTCACCTGCATGC 3'

REV 5' ACGGGAGTGGGCTTACTTTC 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 123 bp

Spans 1 intron

NM_139542 · chr3L

108 1 gene · 2 transcripts

FWD 5' TGGCTACTATGCCGATGTGG 3'

REV 5' CGCATGCAGGTGAACGATTC 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 150 bp

Spans 1 intron