CHRNA5 20 primer pairs found

Human

Show all (including non-specific)

NM_001307945 · chr15

113 Specific

FWD 5' AGACTCTGTCTGGACACCAG 3'

REV 5' CCGATCAAGAACCTGGGCTA 3'

Fwd Tm 60.4°C

Rev Tm 61.1°C

GC 55%

Product 126 bp

Spans 2 introns

NM_001307945 · chr15

112 Specific

FWD 5' AGACTCTGTCTGGACACCAG 3'

REV 5' ATCCGATCAAGAACCTGGGC 3'

Fwd Tm 60.4°C

Rev Tm 61.8°C

GC 55%

Product 128 bp

Spans 2 introns

NM_001307945 · chr15

111 Specific

FWD 5' AGACTCTGTCTGGACACCAG 3'

REV 5' CATCCGATCAAGAACCTGGG 3'

Fwd Tm 60.4°C

Rev Tm 59.9°C

GC 55%

Product 129 bp

Spans 2 introns

NM_000745 · chr15

109 Specific

FWD 5' GGAAGCTGCGCTCGATTCTA 3'

REV 5' ATCCGATCAAGAACCTGGGC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 111 bp

Spans 1 intron

NM_000745 · chr15

109 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' CAGGTGTTCCACAGGACGAA 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 110 bp

Spans 1 intron

NM_000745 · chr15

109 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' TTCAGGTGTTCCACAGGACG 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 112 bp

Spans 1 intron

NM_001307945 · chr15

109 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' CAGGTGTTCCACAGGACGAA 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 110 bp

Spans 1 intron

NM_001307945 · chr15

109 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' TTCAGGTGTTCCACAGGACG 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 112 bp

Spans 1 intron

NM_000745 · chr15

109 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' AGGTGTTCCACAGGACGAAC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 109 bp

Spans 1 intron

NM_001307945 · chr15

109 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' AGGTGTTCCACAGGACGAAC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 109 bp

Spans 1 intron

NM_000745 · chr15

109 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' GTTCCACAGGACGAACCCAT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 105 bp

Spans 1 intron

NM_001307945 · chr15

109 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' GTTCCACAGGACGAACCCAT 3'

Fwd Tm 61.7°C

Rev Tm 61.9°C

GC 55%

Product 105 bp

Spans 1 intron

NM_000745 · chr15

108 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' CCACAGGACGAACCCATCTT 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 102 bp

Spans 1 intron

NM_001307945 · chr15

108 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' CCACAGGACGAACCCATCTT 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 102 bp

Spans 1 intron

NM_000745 · chr15

108 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' TTCCACAGGACGAACCCATC 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 104 bp

Spans 1 intron

NM_001307945 · chr15

108 1 gene · 2 transcripts

FWD 5' GCGCGCAGAGAGGATTATCT 3'

REV 5' TTCCACAGGACGAACCCATC 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 104 bp

Spans 1 intron

NM_001307945 · chr15

107 Specific

FWD 5' AGACTCTGTCTGGACACCAG 3'

REV 5' TCCGATCAAGAACCTGGGCT 3'

Fwd Tm 60.4°C

Rev Tm 63.0°C

GC 55%

Product 127 bp

Spans 2 introns

NM_000745 · chr15

106 Specific

FWD 5' AAACACATTGGAAGCTGCGC 3'

REV 5' ATCCGATCAAGAACCTGGGC 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 50%

Product 120 bp

Spans 1 intron

NM_000745 · chr15

106 Specific

FWD 5' CATTGGAAGCTGCGCTCGAT 3'

REV 5' TCCGATCAAGAACCTGGGCT 3'

Fwd Tm 63.0°C

Rev Tm 63.0°C

GC 55%

Product 114 bp

Spans 1 intron

NM_000745 · chr15

106 Specific

FWD 5' TTGGAAGCTGCGCTCGATTC 3'

REV 5' TCCGATCAAGAACCTGGGCT 3'

Fwd Tm 62.9°C

Rev Tm 63.0°C

GC 55%

Product 112 bp

Spans 1 intron