Cckar 10 primer pairs found

Human

Show all (including non-specific)

NM_000730 · chr4

110 Specific

FWD 5' CGTCCTTCCAAAGAGTGGCA 3'

REV 5' AAGATGTTGGTGACCGTCCG 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 137 bp

Spans 1 intron

NM_000730 · chr4

110 Specific

FWD 5' ACTTCATGGGCACCTCTGTG 3'

REV 5' ACCAGGTAGCAGCAATCACC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 144 bp

Spans 1 intron

NM_000730 · chr4

110 Specific

FWD 5' CGTCCTTCCAAAGAGTGGCA 3'

REV 5' CCGAATCAGCACGGTGATGA 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 105 bp

Spans 1 intron

NM_000730 · chr4

110 Specific

FWD 5' CGTCCTTCCAAAGAGTGGCA 3'

REV 5' TCCGAATCAGCACGGTGATG 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 106 bp

Spans 1 intron

NM_000730 · chr4

109 Specific

FWD 5' CTGTGAACTCGGGCTCGAAA 3'

REV 5' ATCAGCACGGTGATGACCAG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 150 bp

Spans 1 intron

NM_000730 · chr4

109 Specific

FWD 5' CAGTCCTGGCACACATTCCT 3'

REV 5' CAGGTAACACCCATCGCTGT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 195 bp

Spans 2 introns

NM_000730 · chr4

109 Specific

FWD 5' AGTCCTGGCACACATTCCTG 3'

REV 5' CAGGTAACACCCATCGCTGT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 194 bp

Spans 2 introns

NM_000730 · chr4

109 Specific

FWD 5' GTCCTGGCACACATTCCTGT 3'

REV 5' CTGCTGGTGGTGCTAGGTTT 3'

Fwd Tm 62.2°C

Rev Tm 62.2°C

GC 55%

Product 159 bp

Spans 2 introns

NM_000730 · chr4

109 Specific

FWD 5' GTCCTGGCACACATTCCTGT 3'

REV 5' TGCTGGTGGTGCTAGGTTTC 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 158 bp

Spans 2 introns

NM_000730 · chr4

109 Specific

FWD 5' TCCTTCCAAAGAGTGGCAGC 3'

REV 5' CCGAATCAGCACGGTGATGA 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 103 bp

Spans 1 intron