Chrna1 20 primer pairs found

Human

Show all (including non-specific)

NM_001039523 · chr2

116 Specific

FWD 5' GTGCGTCTGAAACAGGGTGA 3'

REV 5' ACACCGCCATAGTCATCTGG 3'

Fwd Tm 62.2°C

Rev Tm 61.4°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001039523 · chr2

116 Specific

FWD 5' TGCGTCTGAAACAGGGTGAC 3'

REV 5' ACACCGCCATAGTCATCTGG 3'

Fwd Tm 62.2°C

Rev Tm 61.4°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001039523 · chr2

116 Specific

FWD 5' GCGTCTGAAACAGGGTGACA 3'

REV 5' ACACCGCCATAGTCATCTGG 3'

Fwd Tm 62.2°C

Rev Tm 61.4°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001039523 · chr2

115 Specific

FWD 5' ATGTGCGTCTGAAACAGGGT 3'

REV 5' ACACCGCCATAGTCATCTGG 3'

Fwd Tm 61.6°C

Rev Tm 61.4°C

GC 50%

Product 142 bp

Spans 2 introns

NM_001039523 · chr2

115 Specific

FWD 5' TGTGCGTCTGAAACAGGGTG 3'

REV 5' ACACCGCCATAGTCATCTGG 3'

Fwd Tm 62.5°C

Rev Tm 61.4°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001039523 · chr2

115 Specific

FWD 5' GTGCGTCTGAAACAGGGTGA 3'

REV 5' CACCGCCATAGTCATCTGGA 3'

Fwd Tm 62.2°C

Rev Tm 61.1°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001039523 · chr2

115 Specific

FWD 5' TGCGTCTGAAACAGGGTGAC 3'

REV 5' CACCGCCATAGTCATCTGGA 3'

Fwd Tm 62.2°C

Rev Tm 61.1°C

GC 55%

Product 138 bp

Spans 2 introns

NM_001039523 · chr2

115 Specific

FWD 5' GCGTCTGAAACAGGGTGACA 3'

REV 5' CACCGCCATAGTCATCTGGA 3'

Fwd Tm 62.2°C

Rev Tm 61.1°C

GC 55%

Product 137 bp

Spans 2 introns

NM_001039523 · chr2

114 Specific

FWD 5' ATGTGCGTCTGAAACAGGGT 3'

REV 5' CACCGCCATAGTCATCTGGA 3'

Fwd Tm 61.6°C

Rev Tm 61.1°C

GC 50%

Product 141 bp

Spans 2 introns

NM_001039523 · chr2

113 Specific

FWD 5' TGTGCGTCTGAAACAGGGTG 3'

REV 5' CACCGCCATAGTCATCTGGA 3'

Fwd Tm 62.5°C

Rev Tm 61.1°C

GC 55%

Product 140 bp

Spans 2 introns

NM_000079 · chr2

109 1 gene · 2 transcripts

FWD 5' GCCATCGAGGGCATCAAGTA 3'

REV 5' GCTAGGGTTCCGATGATGCA 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 149 bp

Spans 1 intron

NM_000079 · chr2

109 1 gene · 2 transcripts

FWD 5' GCCATCGAGGGCATCAAGTA 3'

REV 5' TCCGAGGAGTATGTGGTCCA 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 114 bp

Spans 1 intron

NM_000079 · chr2

109 1 gene · 2 transcripts

FWD 5' AAGACTACAGCAGCGTGGTG 3'

REV 5' GACGCACATTGGTTGTCACG 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 55%

Product 129 bp

Spans 1 intron

NM_000079 · chr2

108 Specific

FWD 5' GCGTCTGAAACAGCAATGGG 3'

REV 5' CGTGATGTGGCCAGTGTACT 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 190 bp

Spans 2 introns

NM_000079 · chr2

108 Specific

FWD 5' AAGACTACAGCAGCGTGGTG 3'

REV 5' CCCATTGCTGTTTCAGACGC 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 144 bp

Spans 1 intron

NM_000079 · chr2

108 1 gene · 2 transcripts

FWD 5' CATCGTCACCCACTTTCCCT 3'

REV 5' GAAGTTGCTCAGGTCTGGCT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 121 bp

Spans 1 intron

NM_000079 · chr2

108 1 gene · 2 transcripts

FWD 5' AGGAGTCTAACAATGCGGCG 3'

REV 5' TGAGTCGACCTGCAAACACG 3'

Fwd Tm 62.1°C

Rev Tm 62.4°C

GC 55%

Product 123 bp

Spans 1 intron

NM_000079 · chr2

108 Specific

FWD 5' GCGTCTGAAACAGCAATGGG 3'

REV 5' CACGTGATGTGGCCAGTGTA 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 192 bp

Spans 2 introns

NM_000079 · chr2

108 1 gene · 2 transcripts

FWD 5' AGGAGTCTAACAATGCGGCG 3'

REV 5' GCTAGGGTTCCGATGATGCA 3'

Fwd Tm 62.1°C

Rev Tm 61.7°C

GC 55%

Product 103 bp

Spans 1 intron

NM_000079 · chr2

108 Specific

FWD 5' GCGTCTGAAACAGCAATGGG 3'

REV 5' ACGTGATGTGGCCAGTGTAC 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 191 bp

Spans 2 introns