Col20a1 10 primer pairs found

Human

Show all (including non-specific)

NM_020882 · chr20

119 Specific

FWD 5' ACGAAGTACAGGGTCCTGGT 3'

REV 5' AGGCCACCATCAGGTCAAAC 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 133 bp

Spans 2 introns

NM_020882 · chr20

118 Specific

FWD 5' AGGGTCCTGGTCTCAGCTAT 3'

REV 5' GAAGGCCACCATCAGGTCAA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 126 bp

Spans 2 introns

NM_020882 · chr20

118 Specific

FWD 5' ACGAAGTACAGGGTCCTGGT 3'

REV 5' GAAGGCCACCATCAGGTCAA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 2 introns

NM_020882 · chr20

118 Specific

FWD 5' ATCTATGCAGCAGGCAGGAG 3'

REV 5' GAAGGCCACCATCAGGTCAA 3'

Fwd Tm 61.5°C

Rev Tm 62.0°C

GC 55%

Product 108 bp

Spans 2 introns

NM_020882 · chr20

118 Specific

FWD 5' AGTACAGGGTCCTGGTCTCA 3'

REV 5' GAAGGCCACCATCAGGTCAA 3'

Fwd Tm 61.5°C

Rev Tm 62.0°C

GC 55%

Product 131 bp

Spans 2 introns

NM_020882 · chr20

117 Specific

FWD 5' AGGGTCCTGGTCTCAGCTAT 3'

REV 5' AGGCCACCATCAGGTCAAAC 3'

Fwd Tm 61.7°C

Rev Tm 62.2°C

GC 55%

Product 124 bp

Spans 2 introns

NM_020882 · chr20

117 Specific

FWD 5' CAGGAGTGAGGCTGTGTCTG 3'

REV 5' GAAGGCCACCATCAGGTCAA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 60%

Product 94 bp

Spans 2 introns

NM_020882 · chr20

117 Specific

FWD 5' GCGTTTCAGCTCCAGATGCT 3'

REV 5' AACAGGAACAGGCAGACACG 3'

Fwd Tm 62.7°C

Rev Tm 62.3°C

GC 55%

Product 127 bp

Spans 2 introns

NM_020882 · chr20

117 Specific

FWD 5' ATCTATGCAGCAGGCAGGAG 3'

REV 5' AGGCCACCATCAGGTCAAAC 3'

Fwd Tm 61.5°C

Rev Tm 62.2°C

GC 55%

Product 106 bp

Spans 2 introns

NM_020882 · chr20

117 Specific

FWD 5' GTTTCAGCTCCAGATGCTGC 3'

REV 5' GAGGAACAGGAACAGGCAGA 3'

Fwd Tm 61.2°C

Rev Tm 61.3°C

GC 55%

Product 129 bp

Spans 2 introns