Col27a1 10 primer pairs found

Human

Show all (including non-specific)

NM_032888 · chr9

120 Specific

FWD 5' GTGTTGGGTCCGATTGGCTA 3'

REV 5' TGGAATCCGGGATCTCCTGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 131 bp

Spans 2 introns

NM_032888 · chr9

120 Specific

FWD 5' TGTTGGGTCCGATTGGCTAC 3'

REV 5' TGGAATCCGGGATCTCCTGA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 130 bp

Spans 2 introns

NM_032888 · chr9

119 Specific

FWD 5' TCAGGAGATCCCGGATTCCA 3'

REV 5' CGGGAAACCCAATGGATCCT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 121 bp

Spans 2 introns

NM_032888 · chr9

119 Specific

FWD 5' GCCAGGAATCGTGGGAGAAA 3'

REV 5' CCATACCACCTGGCATTCCA 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 2 introns

NM_032888 · chr9

119 Specific

FWD 5' AAATCCTGGAGAACTGGGCC 3'

REV 5' TGCCCATCAGTCCCTTCATG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 119 bp

Spans 2 introns

NM_032888 · chr9

119 Specific

FWD 5' CATGAAGGGACTGATGGGCA 3'

REV 5' TGGAATCCGGGATCTCCTGA 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 96 bp

Spans 2 introns

NM_032888 · chr9

119 Specific

FWD 5' AAATCCTGGAGAACTGGGCC 3'

REV 5' TCAGTCCCTTCATGCCCTTG 3'

Fwd Tm 61.8°C

Rev Tm 61.7°C

GC 55%

Product 113 bp

Spans 2 introns

NM_032888 · chr9

119 Specific

FWD 5' CTGGGCAAAGTCGGAGACAA 3'

REV 5' CAGGCTTACCCTTCATGCCT 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 121 bp

Spans 2 introns

NM_032888 · chr9

119 Specific

FWD 5' CAAGGGCATGAAGGGACTGA 3'

REV 5' TGGAATCCGGGATCTCCTGA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 102 bp

Spans 2 introns

NM_032888 · chr9

119 Specific

FWD 5' GTGTTGGGTCCGATTGGCTA 3'

REV 5' ATCCGGGATCTCCTGACACA 3'

Fwd Tm 61.9°C

Rev Tm 62.2°C

GC 55%

Product 127 bp

Spans 2 introns