Col5a2 10 primer pairs found

Human

Show all (including non-specific)

NM_000393 · chr2

120 Specific

FWD 5' AATGCCAGGAGAGAGAGGGA 3'

REV 5' AGCCTGGTATCCCAAGAGGA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 110 bp

Spans 2 introns

NM_000393 · chr2

119 Specific

FWD 5' CACCTGGTTCCAAGGGTGAA 3'

REV 5' TCCCTCTCTCTCCTGGCATT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 90 bp

Spans 2 introns

NM_000393 · chr2

119 Specific

FWD 5' GTGAAGTTGGAGCACCTGGT 3'

REV 5' TCCCTCTCTCTCCTGGCATT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 102 bp

Spans 2 introns

NM_000393 · chr2

119 Specific

FWD 5' CTGGTTCCAAGGGTGAAGCT 3'

REV 5' AGGCATACCATGTGCACCTC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 137 bp

Spans 3 introns

NM_000393 · chr2

119 Specific

FWD 5' TTGGAGCACCTGGTTCCAAG 3'

REV 5' AGGCATACCATGTGCACCTC 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 3 introns

NM_000393 · chr2

119 Specific

FWD 5' TGGTTCCAAGGGTGAAGCTG 3'

REV 5' AGGCATACCATGTGCACCTC 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 3 introns

NM_000393 · chr2

119 Specific

FWD 5' AAGTTGGAGCACCTGGTTCC 3'

REV 5' AGGCATACCATGTGCACCTC 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 3 introns

NM_000393 · chr2

119 Specific

FWD 5' AGGTGAAGTTGGAGCACCTG 3'

REV 5' TCCCTCTCTCTCCTGGCATT 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 104 bp

Spans 2 introns

NM_000393 · chr2

119 Specific

FWD 5' TCAGGGAATTCGAGGCCAAC 3'

REV 5' ACCCTGAATACCATGTGGCC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 97 bp

Spans 2 introns

NM_000393 · chr2

119 Specific

FWD 5' CACCTGGTTCCAAGGGTGAA 3'

REV 5' AGGCATACCATGTGCACCTC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 140 bp

Spans 3 introns