Crlf1 10 primer pairs found

Human

Show all (including non-specific)

NM_004750 · chr19

112 Specific

FWD 5' TGCAGAAGTCGCACAAGACC 3'

REV 5' CAGTTTGGGTTCGGCCTCTG 3'

Fwd Tm 62.5°C

Rev Tm 63.1°C

GC 55%

Product 147 bp

Spans 2 introns

NM_004750 · chr19

110 Specific

FWD 5' CAGAAGTCGCACAAGACCCG 3'

REV 5' CAGTTTGGGTTCGGCCTCTG 3'

Fwd Tm 63.0°C

Rev Tm 63.1°C

GC 60%

Product 145 bp

Spans 2 introns

NM_004750 · chr19

110 Specific

FWD 5' GCAGAAGTCGCACAAGACCC 3'

REV 5' CAGTTTGGGTTCGGCCTCTG 3'

Fwd Tm 63.3°C

Rev Tm 63.1°C

GC 60%

Product 146 bp

Spans 2 introns

NM_004750 · chr19

110 Specific

FWD 5' CATCAGCTGCTGGTCCAAGA 3'

REV 5' TGTTGTCCTGGCCATACCAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 128 bp

Spans 1 intron

NM_004750 · chr19

110 Specific

FWD 5' CATCAGCTGCTGGTCCAAGA 3'

REV 5' GTGTTGTCCTGGCCATACCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 129 bp

Spans 1 intron

NM_004750 · chr19

110 Specific

FWD 5' CATCAGCTGCTGGTCCAAGA 3'

REV 5' TGTGTTGTCCTGGCCATACC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 130 bp

Spans 1 intron

NM_004750 · chr19

110 Specific

FWD 5' TACCGAGTGGAGGACAGTGT 3'

REV 5' CCGGCTTTCTTGGAGCCATA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 149 bp

Spans 1 intron

NM_004750 · chr19

110 Specific

FWD 5' ATGAAGGACTTGACCTGCCG 3'

REV 5' TGTTGTCCTGGCCATACCAC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 106 bp

Spans 1 intron

NM_004750 · chr19

110 Specific

FWD 5' ATGAAGGACTTGACCTGCCG 3'

REV 5' GTGTTGTCCTGGCCATACCA 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 107 bp

Spans 1 intron

NM_004750 · chr19

110 Specific

FWD 5' ATGAAGGACTTGACCTGCCG 3'

REV 5' TGTGTTGTCCTGGCCATACC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 108 bp

Spans 1 intron