Cyp27b1 10 primer pairs found

Human

Show all (including non-specific)

NM_000785 · chr12

118 Specific

FWD 5' CGGTGGTCAAGGAAGTGCTA 3'

REV 5' GGGTCCCTTGAAGTGGCATA 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 142 bp

Spans 2 introns

NM_000785 · chr12

118 Specific

FWD 5' CGGTGGTCAAGGAAGTGCTA 3'

REV 5' AGTGACACAGAGTGACCAGC 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 123 bp

Spans 2 introns

NM_000785 · chr12

113 Specific

FWD 5' TGAAGGCGGTGGTCAAGGAA 3'

REV 5' AGGGTCCCTTGAAGTGGCAT 3'

Fwd Tm 63.7°C

Rev Tm 63.4°C

GC 55%

Product 149 bp

Spans 2 introns

NM_000785 · chr12

113 Specific

FWD 5' TGAAGGCGGTGGTCAAGGAA 3'

REV 5' TGACACAGAGTGACCAGCGT 3'

Fwd Tm 63.7°C

Rev Tm 63.2°C

GC 55%

Product 127 bp

Spans 2 introns

NM_000785 · chr12

113 Specific

FWD 5' AAGGCGGTGGTCAAGGAAGT 3'

REV 5' AGGGTCCCTTGAAGTGGCAT 3'

Fwd Tm 63.7°C

Rev Tm 63.4°C

GC 55%

Product 147 bp

Spans 2 introns

NM_000785 · chr12

113 Specific

FWD 5' AAGGCGGTGGTCAAGGAAGT 3'

REV 5' TGACACAGAGTGACCAGCGT 3'

Fwd Tm 63.7°C

Rev Tm 63.2°C

GC 55%

Product 125 bp

Spans 2 introns

NM_000785 · chr12

112 Specific

FWD 5' GAAGGCGGTGGTCAAGGAAG 3'

REV 5' TGACACAGAGTGACCAGCGT 3'

Fwd Tm 62.9°C

Rev Tm 63.2°C

GC 60%

Product 126 bp

Spans 2 introns

NM_000785 · chr12

112 Specific

FWD 5' CGGTGGTCAAGGAAGTGCTA 3'

REV 5' GTGACACAGAGTGACCAGCG 3'

Fwd Tm 61.5°C

Rev Tm 62.6°C

GC 55%

Product 122 bp

Spans 2 introns

NM_000785 · chr12

112 Specific

FWD 5' CGGTGGTCAAGGAAGTGCTA 3'

REV 5' TGACACAGAGTGACCAGCGT 3'

Fwd Tm 61.5°C

Rev Tm 63.2°C

GC 55%

Product 121 bp

Spans 2 introns

NM_000785 · chr12

111 Specific

FWD 5' GAAGGCGGTGGTCAAGGAAG 3'

REV 5' AGGGTCCCTTGAAGTGGCAT 3'

Fwd Tm 62.9°C

Rev Tm 63.4°C

GC 60%

Product 148 bp

Spans 2 introns