DACT1 20 primer pairs found

Human

Show all (including non-specific)

NM_001079520 · chr14

108 1 gene · 2 transcripts

FWD 5' AGGCGACCTTGAGTCTCTCA 3'

REV 5' ACAGCATGAAGTGGACTGGG 3'

Fwd Tm 62.2°C

Rev Tm 61.9°C

GC 55%

Product 124 bp

Spans 1 intron

NM_001079520 · chr14

108 1 gene · 2 transcripts

FWD 5' AGGCGACCTTGAGTCTCTCA 3'

REV 5' CAGCCACAGCATGAAGTGGA 3'

Fwd Tm 62.2°C

Rev Tm 62.4°C

GC 55%

Product 129 bp

Spans 1 intron

NM_001079520 · chr14

108 1 gene · 2 transcripts

FWD 5' AGGCGACCTTGAGTCTCTCA 3'

REV 5' GCCACAGCATGAAGTGGACT 3'

Fwd Tm 62.2°C

Rev Tm 62.4°C

GC 55%

Product 127 bp

Spans 1 intron

NM_001079520 · chr14

108 1 gene · 2 transcripts

FWD 5' AGGCGACCTTGAGTCTCTCA 3'

REV 5' AGCCACAGCATGAAGTGGAC 3'

Fwd Tm 62.2°C

Rev Tm 62.4°C

GC 55%

Product 128 bp

Spans 1 intron

NM_016651 · chr14

107 Specific

FWD 5' GTTGTCATTCCAGCACCTGC 3'

REV 5' GGTCTTCACAGTGGCCTTCT 3'

Fwd Tm 61.4°C

Rev Tm 61.6°C

GC 55%

Product 123 bp

Spans 1 intron

NM_016651 · chr14

107 Specific

FWD 5' GTTGTCATTCCAGCACCTGC 3'

REV 5' TGGTCTTCACAGTGGCCTTC 3'

Fwd Tm 61.4°C

Rev Tm 61.8°C

GC 55%

Product 124 bp

Spans 1 intron

NM_016651 · chr14

107 Specific

FWD 5' GTTGTCATTCCAGCACCTGC 3'

REV 5' CTGGTCTTCACAGTGGCCTT 3'

Fwd Tm 61.4°C

Rev Tm 61.8°C

GC 55%

Product 125 bp

Spans 1 intron

NM_001079520 · chr14

107 1 gene · 2 transcripts

FWD 5' AGGCGACCTTGAGTCTCTCA 3'

REV 5' CAGCATGAAGTGGACTGGGA 3'

Fwd Tm 62.2°C

Rev Tm 61.6°C

GC 55%

Product 123 bp

Spans 1 intron

NM_001079520 · chr14

107 1 gene · 2 transcripts

FWD 5' AGGCGACCTTGAGTCTCTCA 3'

REV 5' ACAGCCACAGCATGAAGTGG 3'

Fwd Tm 62.2°C

Rev Tm 62.7°C

GC 55%

Product 130 bp

Spans 1 intron

NM_001079520 · chr14

107 1 gene · 2 transcripts

FWD 5' TTGGAGGCGACCTTGAGTCT 3'

REV 5' ACAGCCACAGCATGAAGTGG 3'

Fwd Tm 62.8°C

Rev Tm 62.7°C

GC 55%

Product 134 bp

Spans 1 intron

NM_016651 · chr14

106 Specific

FWD 5' TTGGAGGCGACCTTGAGTCT 3'

REV 5' TGAGGCCTGGTCTTCACAGT 3'

Fwd Tm 62.8°C

Rev Tm 62.9°C

GC 55%

Product 99 bp

Spans 1 intron

NM_016651 · chr14

106 Specific

FWD 5' AGGCGACCTTGAGTCTCTCA 3'

REV 5' TTGTGGTGTGGAGAGGGAAC 3'

Fwd Tm 62.2°C

Rev Tm 61.5°C

GC 55%

Product 128 bp

Spans 1 intron

NM_016651 · chr14

106 Specific

FWD 5' AGGCGACCTTGAGTCTCTCA 3'

REV 5' CTGAGGCCTGGTCTTCACAG 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 96 bp

Spans 1 intron

NM_001079520 · chr14

106 1 gene · 2 transcripts

FWD 5' AGTCTCTCAGATGGTTGCCC 3'

REV 5' CACAGCCACAGCATGAAGTG 3'

Fwd Tm 61.1°C

Rev Tm 61.4°C

GC 55%

Product 120 bp

Spans 1 intron

NM_001079520 · chr14

106 1 gene · 2 transcripts

FWD 5' TTGGAGGCGACCTTGAGTCT 3'

REV 5' GCCACAGCATGAAGTGGACT 3'

Fwd Tm 62.8°C

Rev Tm 62.4°C

GC 55%

Product 131 bp

Spans 1 intron

NM_001079520 · chr14

106 1 gene · 2 transcripts

FWD 5' TTGGAGGCGACCTTGAGTCT 3'

REV 5' AGCCACAGCATGAAGTGGAC 3'

Fwd Tm 62.8°C

Rev Tm 62.4°C

GC 55%

Product 132 bp

Spans 1 intron

NM_016651 · chr14

106 Specific

FWD 5' AGGCGACCTTGAGTCTCTCA 3'

REV 5' GAGGCCTGGTCTTCACAGTG 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 94 bp

Spans 1 intron

NM_016651 · chr14

106 Specific

FWD 5' TTGTCATTCCAGCACCTGCT 3'

REV 5' TGGTCTTCACAGTGGCCTTC 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 50%

Product 123 bp

Spans 1 intron

NM_016651 · chr14

106 Specific

FWD 5' TGTCATTCCAGCACCTGCTT 3'

REV 5' TGGTCTTCACAGTGGCCTTC 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 50%

Product 122 bp

Spans 1 intron

NM_016651 · chr14

106 Specific

FWD 5' AGTCTCTCAGATGGTTGCCC 3'

REV 5' TTGTGGTGTGGAGAGGGAAC 3'

Fwd Tm 61.1°C

Rev Tm 61.5°C

GC 55%

Product 117 bp

Spans 1 intron