DEF6 10 primer pairs found

Human

Show all (including non-specific)

NM_022047 · chr6

112 Specific

FWD 5' CTGACGAGGATGTGGAGGCT 3'

REV 5' AAGCCTGAGAAGGAGCTGCT 3'

Fwd Tm 63.4°C

Rev Tm 63.3°C

GC 60%

Product 145 bp

Spans 2 introns

NM_022047 · chr6

111 Specific

FWD 5' CAGAGGGCTGACGAGGATGT 3'

REV 5' TGAGAAGGAGCTGCTGGTGA 3'

Fwd Tm 63.4°C

Rev Tm 62.9°C

GC 60%

Product 147 bp

Spans 2 introns

NM_022047 · chr6

111 Specific

FWD 5' AGAGGGCTGACGAGGATGTG 3'

REV 5' TGAGAAGGAGCTGCTGGTGA 3'

Fwd Tm 63.4°C

Rev Tm 62.9°C

GC 60%

Product 146 bp

Spans 2 introns

NM_022047 · chr6

111 Specific

FWD 5' CTGACGAGGATGTGGAGGCT 3'

REV 5' TGAGAAGGAGCTGCTGGTGA 3'

Fwd Tm 63.4°C

Rev Tm 62.9°C

GC 60%

Product 140 bp

Spans 2 introns

NM_022047 · chr6

111 Specific

FWD 5' CTGACGAGGATGTGGAGGCT 3'

REV 5' TGGAAGCCTGAGAAGGAGCT 3'

Fwd Tm 63.4°C

Rev Tm 62.7°C

GC 60%

Product 148 bp

Spans 2 introns

NM_022047 · chr6

111 Specific

FWD 5' TGACGAGGATGTGGAGGCTG 3'

REV 5' AAGCCTGAGAAGGAGCTGCT 3'

Fwd Tm 63.6°C

Rev Tm 63.3°C

GC 60%

Product 144 bp

Spans 2 introns

NM_022047 · chr6

110 Specific

FWD 5' TGACGAGGATGTGGAGGCTG 3'

REV 5' TGAGAAGGAGCTGCTGGTGA 3'

Fwd Tm 63.6°C

Rev Tm 62.9°C

GC 60%

Product 139 bp

Spans 2 introns

NM_022047 · chr6

110 Specific

FWD 5' TGACGAGGATGTGGAGGCTG 3'

REV 5' TGGAAGCCTGAGAAGGAGCT 3'

Fwd Tm 63.6°C

Rev Tm 62.7°C

GC 60%

Product 147 bp

Spans 2 introns

NM_022047 · chr6

110 Specific

FWD 5' TCTGTGCGAATCGCTCAGAC 3'

REV 5' CATCTCCTGCTGCAGCTCTT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 1 intron

NM_022047 · chr6

110 Specific

FWD 5' CTCAAGGTGCTGTCCCACAA 3'

REV 5' CACAGGGCCGTCATCATCAT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 99 bp

Spans 1 intron