No gene-specific primers found for DEFA1B. Showing all 60 primers including those that may amplify other genes. Consider validating with NCBI Primer-BLAST.

DEFA1B 60 primer pairs found

Human

Show all (including non-specific)

NM_001302265 · chr8

117 3 genes · 4 transcripts

FWD 5' ACCCTGCCTAGCTAGAGGAT 3'

REV 5' CAGGAGAATGGCAGCAAGGA 3'

Fwd Tm 61.5°C

Rev Tm 62.0°C

GC 55%

Product 114 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

117 3 genes · 4 transcripts

FWD 5' ACCCTGCCTAGCTAGAGGAT 3'

REV 5' CAGGAGAATGGCAGCAAGGA 3'

Fwd Tm 61.5°C

Rev Tm 62.0°C

GC 55%

Product 114 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

117 3 genes · 4 transcripts

FWD 5' ACCCTGCCTAGCTAGAGGAT 3'

REV 5' CAGGAGAATGGCAGCAAGGA 3'

Fwd Tm 61.5°C

Rev Tm 62.0°C

GC 55%

Product 114 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' GACAGAGGACTGCTGTCTGC 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 60%

Product 147 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' GACAGAGGACTGCTGTCTGC 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 60%

Product 147 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' GACAGAGGACTGCTGTCTGC 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 60%

Product 147 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' GGACAGAGGACTGCTGTCTG 3'

REV 5' CAGGAGAATGGCAGCAAGGA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 60%

Product 146 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' GGACAGAGGACTGCTGTCTG 3'

REV 5' CAGGAGAATGGCAGCAAGGA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 60%

Product 146 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' GGACAGAGGACTGCTGTCTG 3'

REV 5' CAGGAGAATGGCAGCAAGGA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 60%

Product 146 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' GACAGAGGACTGCTGTCTGC 3'

REV 5' CAGGAGAATGGCAGCAAGGA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 60%

Product 145 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' ACCCTGCCTAGCTAGAGGAT 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 61.5°C

Rev Tm 62.3°C

GC 55%

Product 116 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' GACAGAGGACTGCTGTCTGC 3'

REV 5' CAGGAGAATGGCAGCAAGGA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 60%

Product 145 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' ACCCTGCCTAGCTAGAGGAT 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 61.5°C

Rev Tm 62.3°C

GC 55%

Product 116 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' GACAGAGGACTGCTGTCTGC 3'

REV 5' CAGGAGAATGGCAGCAAGGA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 60%

Product 145 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' ACCCTGCCTAGCTAGAGGAT 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 61.5°C

Rev Tm 62.3°C

GC 55%

Product 116 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' TCACCCTGCCTAGCTAGAGG 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 60%

Product 118 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' CACCCTGCCTAGCTAGAGGA 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 60%

Product 117 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' TCACCCTGCCTAGCTAGAGG 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 60%

Product 118 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' CACCCTGCCTAGCTAGAGGA 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 60%

Product 117 bp

Spans 2 introns

NCBI BLAST

NM_001302265 · chr8

116 3 genes · 4 transcripts

FWD 5' TCACCCTGCCTAGCTAGAGG 3'

REV 5' ACCAGGAGAATGGCAGCAAG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 60%

Product 118 bp

Spans 2 introns

NCBI BLAST

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