DEFA4 10 primer pairs found

Human

Show all (including non-specific)

NM_001925 · chr8

107 Specific

FWD 5' GGAACACAGGACTGCTGTCT 3'

REV 5' ATCACCTCTTGCCTGGAGTG 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 148 bp

Spans 1 intron

NM_001925 · chr8

107 Specific

FWD 5' GGAACACAGGACTGCTGTCT 3'

REV 5' CATCACCTCTTGCCTGGAGT 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001925 · chr8

107 Specific

FWD 5' TGGAACACAGGACTGCTGTC 3'

REV 5' ATCACCTCTTGCCTGGAGTG 3'

Fwd Tm 61.7°C

Rev Tm 61.4°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001925 · chr8

107 Specific

FWD 5' TGGAACACAGGACTGCTGTC 3'

REV 5' CATCACCTCTTGCCTGGAGT 3'

Fwd Tm 61.7°C

Rev Tm 61.4°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001925 · chr8

107 Specific

FWD 5' CTGGAACACAGGACTGCTGT 3'

REV 5' ATCACCTCTTGCCTGGAGTG 3'

Fwd Tm 61.8°C

Rev Tm 61.4°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001925 · chr8

106 Specific

FWD 5' AGCTTGAGGATCTGTCACCC 3'

REV 5' TCATCACCTCTTGCCTGGAG 3'

Fwd Tm 61.1°C

Rev Tm 61.1°C

GC 55%

Product 108 bp

Spans 1 intron

NM_001925 · chr8

106 Specific

FWD 5' AGCTTGAGGATCTGTCACCC 3'

REV 5' CTCATCACCTCTTGCCTGGA 3'

Fwd Tm 61.1°C

Rev Tm 61.1°C

GC 55%

Product 109 bp

Spans 1 intron

NM_001925 · chr8

106 Specific

FWD 5' AGCTTGAGGATCTGTCACCC 3'

REV 5' TGGAGCCTCATCACCTCTTG 3'

Fwd Tm 61.1°C

Rev Tm 61.1°C

GC 55%

Product 115 bp

Spans 1 intron

NM_001925 · chr8

106 Specific

FWD 5' AACACAGGACTGCTGTCTGC 3'

REV 5' CAGCGAGGAGGGCGATAATC 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 90 bp

Spans 1 intron

NM_001925 · chr8

106 Specific

FWD 5' AACACAGGACTGCTGTCTGC 3'

REV 5' CCTCATCACCTCTTGCCTGG 3'

Fwd Tm 62.3°C

Rev Tm 62.2°C

GC 55%

Product 150 bp

Spans 1 intron