DEFA5 10 primer pairs found

Human

Show all (including non-specific)

NM_021010 · chr8

109 Specific

FWD 5' AGGCTGAGTCACTCCAGGAA 3'

REV 5' AATAGCAGGTGGCTCTTGCC 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 147 bp

Spans 1 intron

NM_021010 · chr8

109 Specific

FWD 5' AGGCTGAGTCACTCCAGGAA 3'

REV 5' GGCAATAGCAGGTGGCTCTT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 150 bp

Spans 1 intron

NM_021010 · chr8

108 Specific

FWD 5' TCAGGTTCTCAGGCAAGAGC 3'

REV 5' TAGAGGCGGCCACTGATTTC 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 55%

Product 98 bp

Spans 1 intron

NM_021010 · chr8

108 Specific

FWD 5' TCAGGTTCTCAGGCAAGAGC 3'

REV 5' GTAGAGGCGGCCACTGATTT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 99 bp

Spans 1 intron

NM_021010 · chr8

108 Specific

FWD 5' GGCTACAACCCAGAAGCAGT 3'

REV 5' AATAGCAGGTGGCTCTTGCC 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 116 bp

Spans 1 intron

NM_021010 · chr8

108 Specific

FWD 5' GGCTACAACCCAGAAGCAGT 3'

REV 5' GGCAATAGCAGGTGGCTCTT 3'

Fwd Tm 61.9°C

Rev Tm 62.3°C

GC 55%

Product 119 bp

Spans 1 intron

NM_021010 · chr8

107 Specific

FWD 5' ACCTCAGGTTCTCAGGCAAG 3'

REV 5' CGGCCACTGATTTCACACAC 3'

Fwd Tm 61.3°C

Rev Tm 61.3°C

GC 55%

Product 95 bp

Spans 1 intron

NM_021010 · chr8

107 Specific

FWD 5' TCAGGTTCTCAGGCAAGAGC 3'

REV 5' CGGCCACTGATTTCACACAC 3'

Fwd Tm 61.6°C

Rev Tm 61.3°C

GC 55%

Product 92 bp

Spans 1 intron

NM_021010 · chr8

107 Specific

FWD 5' ACCTCAGGTTCTCAGGCAAG 3'

REV 5' TAGAGGCGGCCACTGATTTC 3'

Fwd Tm 61.3°C

Rev Tm 61.7°C

GC 55%

Product 101 bp

Spans 1 intron

NM_021010 · chr8

107 Specific

FWD 5' GCTGATGAGGCTACAACCCA 3'

REV 5' AATAGCAGGTGGCTCTTGCC 3'

Fwd Tm 61.7°C

Rev Tm 62.3°C

GC 55%

Product 124 bp

Spans 1 intron