DEFB121 20 primer pairs found

Human

Show all (including non-specific)

NM_001171832 · chr20

109 Specific

FWD 5' AAGCCAGAAGAGCCTCAAGG 3'

REV 5' CATGTTGTTCTGCACCTGCC 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 106 bp

Spans 1 intron

NM_001171832 · chr20

107 Specific

FWD 5' AGCCAGAAGAGCCTCAAGGA 3'

REV 5' TTGTTCTGCACCTGCCTGAC 3'

Fwd Tm 62.7°C

Rev Tm 62.6°C

GC 55%

Product 101 bp

Spans 1 intron

NM_001171832 · chr20

107 Specific

FWD 5' AGCCAGAAGAGCCTCAAGGA 3'

REV 5' GTTGTTCTGCACCTGCCTGA 3'

Fwd Tm 62.7°C

Rev Tm 62.6°C

GC 55%

Product 102 bp

Spans 1 intron

NM_001011878 · chr20

107 Specific

FWD 5' ACTGTTACTCTGCTCCTGGC 3'

REV 5' TCCACACAGCACTTAGCCTC 3'

Fwd Tm 61.3°C

Rev Tm 61.5°C

GC 55%

Product 134 bp

Spans 1 intron

NM_001171832 · chr20

107 Specific

FWD 5' AGCCAGAAGAGCCTCAAGGA 3'

REV 5' GTTCTGCACCTGCCTGACTT 3'

Fwd Tm 62.7°C

Rev Tm 62.4°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001171832 · chr20

107 Specific

FWD 5' AGCCAGAAGAGCCTCAAGGA 3'

REV 5' TTCTGCACCTGCCTGACTTG 3'

Fwd Tm 62.7°C

Rev Tm 62.4°C

GC 55%

Product 98 bp

Spans 1 intron

NM_001011878 · chr20

107 Specific

FWD 5' CTGTTACTCTGCTCCTGGCC 3'

REV 5' ACTTGGGATCCACACAGCAC 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 60%

Product 141 bp

Spans 1 intron

NM_001011878 · chr20

107 Specific

FWD 5' ACTGTTACTCTGCTCCTGGC 3'

REV 5' CCACACAGCACTTAGCCTCA 3'

Fwd Tm 61.3°C

Rev Tm 61.8°C

GC 55%

Product 133 bp

Spans 1 intron

NM_001171832 · chr20

107 Specific

FWD 5' AAGCCAGAAGAGCCTCAAGG 3'

REV 5' TTCTGCACCTGCCTGACTTG 3'

Fwd Tm 61.7°C

Rev Tm 62.4°C

GC 55%

Product 99 bp

Spans 1 intron

NM_001011878 · chr20

107 Specific

FWD 5' ACTGTTACTCTGCTCCTGGC 3'

REV 5' CTTGGGATCCACACAGCACT 3'

Fwd Tm 61.3°C

Rev Tm 61.9°C

GC 55%

Product 141 bp

Spans 1 intron

NM_001171832 · chr20

107 Specific

FWD 5' AAGCCAGAAGAGCCTCAAGG 3'

REV 5' GTTCTGCACCTGCCTGACTT 3'

Fwd Tm 61.7°C

Rev Tm 62.4°C

GC 55%

Product 100 bp

Spans 1 intron

NM_001011878 · chr20

106 Specific

FWD 5' CTGTTACTCTGCTCCTGGCC 3'

REV 5' CTTGGGATCCACACAGCACT 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 60%

Product 140 bp

Spans 1 intron

NM_001171832 · chr20

106 Specific

FWD 5' AGCCAGAAGAGCCTCAAGGA 3'

REV 5' TGTTGTTCTGCACCTGCCTG 3'

Fwd Tm 62.7°C

Rev Tm 62.9°C

GC 55%

Product 103 bp

Spans 1 intron

NM_001011878 · chr20

106 Specific

FWD 5' AGAAGCCTCCTATCCCTCCC 3'

REV 5' TTCTGCACCTGCCTGACTTG 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 60%

Product 130 bp

Spans 1 intron

NM_001011878 · chr20

106 Specific

FWD 5' GAAGCCTCCTATCCCTCCCT 3'

REV 5' TTCTGCACCTGCCTGACTTG 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 60%

Product 129 bp

Spans 1 intron

NM_001011878 · chr20

106 Specific

FWD 5' AGAAGCCTCCTATCCCTCCC 3'

REV 5' GTTCTGCACCTGCCTGACTT 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 60%

Product 131 bp

Spans 1 intron

NM_001011878 · chr20

106 Specific

FWD 5' GAAGCCTCCTATCCCTCCCT 3'

REV 5' GTTCTGCACCTGCCTGACTT 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 60%

Product 130 bp

Spans 1 intron

NM_001171832 · chr20

106 Specific

FWD 5' CCAGAAGAGCCTCAAGGAGG 3'

REV 5' CATGTTGTTCTGCACCTGCC 3'

Fwd Tm 61.6°C

Rev Tm 61.7°C

GC 60%

Product 103 bp

Spans 1 intron

NM_001011878 · chr20

106 Specific

FWD 5' CTGTTACTCTGCTCCTGGCC 3'

REV 5' CCACACAGCACTTAGCCTCA 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 60%

Product 132 bp

Spans 1 intron

NM_001171832 · chr20

106 Specific

FWD 5' GCCTCAAGGAGGAAAGAGGT 3'

REV 5' CATGTTGTTCTGCACCTGCC 3'

Fwd Tm 61.1°C

Rev Tm 61.7°C

GC 55%

Product 95 bp

Spans 1 intron