DEFB127 10 primer pairs found

Human

Show all (including non-specific)

NM_139074 · chr20

103 Specific

FWD 5' CTGCTGTTCCAGAAACCCAC 3'

REV 5' TTCACATAGTGCCTCAGGCA 3'

Fwd Tm 60.8°C

Rev Tm 60.9°C

GC 55%

Product 117 bp

Spans 1 intron

NM_139074 · chr20

103 Specific

FWD 5' GCTGTTCCAGAAACCCACAG 3'

REV 5' TTCACATAGTGCCTCAGGCA 3'

Fwd Tm 60.8°C

Rev Tm 60.9°C

GC 55%

Product 115 bp

Spans 1 intron

NM_139074 · chr20

100 Specific

FWD 5' TCTGCTGTTCCAGAAACCCA 3'

REV 5' TTCACATAGTGCCTCAGGCA 3'

Fwd Tm 61.0°C

Rev Tm 60.9°C

GC 50%

Product 118 bp

Spans 1 intron

NM_139074 · chr20

100 Specific

FWD 5' CCAGAAACCCACAGTAACCG 3'

REV 5' TTCACATAGTGCCTCAGGCA 3'

Fwd Tm 60.3°C

Rev Tm 60.9°C

GC 55%

Product 109 bp

Spans 1 intron

NM_139074 · chr20

100 Specific

FWD 5' TGCTGTTCCAGAAACCCACA 3'

REV 5' TTCACATAGTGCCTCAGGCA 3'

Fwd Tm 61.6°C

Rev Tm 60.9°C

GC 50%

Product 116 bp

Spans 1 intron

NM_139074 · chr20

99 Specific

FWD 5' CCAGAAACCCACAGTAACCG 3'

REV 5' TGAGGCAACAGTATCTCCCA 3'

Fwd Tm 60.3°C

Rev Tm 60.0°C

GC 55%

Product 131 bp

Spans 1 intron

NM_139074 · chr20

99 Specific

FWD 5' CTGCTGTTCCAGAAACCCAC 3'

REV 5' TGAGGCAACAGTATCTCCCA 3'

Fwd Tm 60.8°C

Rev Tm 60.0°C

GC 55%

Product 139 bp

Spans 1 intron

NM_139074 · chr20

99 Specific

FWD 5' GCTGTTCCAGAAACCCACAG 3'

REV 5' TGAGGCAACAGTATCTCCCA 3'

Fwd Tm 60.8°C

Rev Tm 60.0°C

GC 55%

Product 137 bp

Spans 1 intron

NM_139074 · chr20

97 Specific

FWD 5' CCAGAAACCCACAGTAACCG 3'

REV 5' TTTCACATAGTGCCTCAGGC 3'

Fwd Tm 60.3°C

Rev Tm 59.6°C

GC 55%

Product 110 bp

Spans 1 intron

NM_139074 · chr20

97 Specific

FWD 5' CTGCTGTTCCAGAAACCCAC 3'

REV 5' TTTCACATAGTGCCTCAGGC 3'

Fwd Tm 60.8°C

Rev Tm 59.6°C

GC 55%

Product 118 bp

Spans 1 intron