No gene-specific primers found for DEFB130B. Showing all 20 primers including those that may amplify other genes. Consider validating with NCBI Primer-BLAST.

DEFB130B 20 primer pairs found

Human

Show all (including non-specific)

NM_001195257 · chr8

89 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' ATAGGGCTCAAGTATCCACC 3'

Fwd Tm 58.1°C

Rev Tm 58.1°C

GC 50%

Product 150 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

89 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' ATAGGGCTCAAGTATCCACC 3'

Fwd Tm 58.1°C

Rev Tm 58.1°C

GC 50%

Product 150 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

89 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' GGCTCAAGTATCCACCACCT 3'

Fwd Tm 58.1°C

Rev Tm 61.2°C

GC 50%

Product 146 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

89 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' AGGGCTCAAGTATCCACCAC 3'

Fwd Tm 58.1°C

Rev Tm 61.2°C

GC 50%

Product 148 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

89 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' GGCTCAAGTATCCACCACCT 3'

Fwd Tm 58.1°C

Rev Tm 61.2°C

GC 50%

Product 146 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

89 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' AGGGCTCAAGTATCCACCAC 3'

Fwd Tm 58.1°C

Rev Tm 61.2°C

GC 50%

Product 148 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

88 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' GCTCAAGTATCCACCACCTT 3'

Fwd Tm 58.1°C

Rev Tm 59.0°C

GC 50%

Product 145 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

88 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' GCTCAAGTATCCACCACCTT 3'

Fwd Tm 58.1°C

Rev Tm 59.0°C

GC 50%

Product 145 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

88 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' TAGGGCTCAAGTATCCACCA 3'

Fwd Tm 58.1°C

Rev Tm 59.5°C

GC 50%

Product 149 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

88 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' TAGGGCTCAAGTATCCACCA 3'

Fwd Tm 58.1°C

Rev Tm 59.5°C

GC 50%

Product 149 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

86 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' GGTATCATCTAGTGTGCTGC 3'

Fwd Tm 58.1°C

Rev Tm 57.3°C

GC 50%

Product 90 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

86 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' GGTATCATCTAGTGTGCTGC 3'

Fwd Tm 58.1°C

Rev Tm 57.3°C

GC 50%

Product 90 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

85 2 genes · 2 transcripts

FWD 5' CTGTTCTCCTCCTCTTTGTG 3'

REV 5' GGTATCATCTAGTGTGCTGC 3'

Fwd Tm 57.1°C

Rev Tm 57.3°C

GC 50%

Product 125 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

85 2 genes · 2 transcripts

FWD 5' CTGTTCTCCTCCTCTTTGTG 3'

REV 5' GGTATCATCTAGTGTGCTGC 3'

Fwd Tm 57.1°C

Rev Tm 57.3°C

GC 50%

Product 125 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

85 2 genes · 2 transcripts

FWD 5' GTTCTCCTCCTCTTTGTGAC 3'

REV 5' GGTATCATCTAGTGTGCTGC 3'

Fwd Tm 57.1°C

Rev Tm 57.3°C

GC 50%

Product 123 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

85 2 genes · 2 transcripts

FWD 5' GTTCTCCTCCTCTTTGTGAC 3'

REV 5' GGTATCATCTAGTGTGCTGC 3'

Fwd Tm 57.1°C

Rev Tm 57.3°C

GC 50%

Product 123 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

84 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' GGGCTCAAGTATCCACCACC 3'

Fwd Tm 58.1°C

Rev Tm 62.2°C

GC 50%

Product 147 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

84 2 genes · 2 transcripts

FWD 5' GGAAAGACTGGCGTTATTCC 3'

REV 5' GGGCTCAAGTATCCACCACC 3'

Fwd Tm 58.1°C

Rev Tm 62.2°C

GC 50%

Product 147 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

84 2 genes · 2 transcripts

FWD 5' CTGTTCTCCTCCTCTTTGTG 3'

REV 5' GCTGCATAGTTTGTCTCTGC 3'

Fwd Tm 57.1°C

Rev Tm 58.6°C

GC 50%

Product 110 bp

Spans 1 intron

NCBI BLAST

NM_001195257 · chr8

84 2 genes · 2 transcripts

FWD 5' CTGTTCTCCTCCTCTTTGTG 3'

REV 5' GCTGCATAGTTTGTCTCTGC 3'

Fwd Tm 57.1°C

Rev Tm 58.6°C

GC 50%

Product 110 bp

Spans 1 intron

NCBI BLAST