DEFB134 10 primer pairs found

Human

Show all (including non-specific)

NM_001302695 · chr8

109 Specific

FWD 5' TCCTTTGGGATCCAGTGCTG 3'

REV 5' GACACAGCACTCCAGCTGAA 3'

Fwd Tm 61.7°C

Rev Tm 62.0°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001302695 · chr8

108 Specific

FWD 5' GAGTGAGCATAGCACACCGA 3'

REV 5' ATACCTGCCAGCACTGGATC 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 125 bp

Spans 1 intron

NM_001302695 · chr8

108 Specific

FWD 5' AGAGTGAGCATAGCACACCG 3'

REV 5' ATACCTGCCAGCACTGGATC 3'

Fwd Tm 61.5°C

Rev Tm 61.5°C

GC 55%

Product 126 bp

Spans 1 intron

NM_001302695 · chr8

108 Specific

FWD 5' AGAGTGAGCATAGCACACCG 3'

REV 5' CAGCACTGGATCCCAAAGGA 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001302695 · chr8

108 Specific

FWD 5' GAGTGAGCATAGCACACCGA 3'

REV 5' CAGCACTGGATCCCAAAGGA 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 117 bp

Spans 1 intron

NM_001302695 · chr8

108 Specific

FWD 5' GATCCAGTGCTGGCAGGTAT 3'

REV 5' GACACAGCACTCCAGCTGAA 3'

Fwd Tm 61.5°C

Rev Tm 62.0°C

GC 55%

Product 138 bp

Spans 1 intron

NM_001302695 · chr8

107 Specific

FWD 5' TCCTTTGGGATCCAGTGCTG 3'

REV 5' TTGACACAGCACTCCAGCTG 3'

Fwd Tm 61.7°C

Rev Tm 62.3°C

GC 55%

Product 148 bp

Spans 1 intron

NM_001302695 · chr8

107 Specific

FWD 5' TAGCACACCGATGTCAGACC 3'

REV 5' ATACCTGCCAGCACTGGATC 3'

Fwd Tm 61.3°C

Rev Tm 61.5°C

GC 55%

Product 116 bp

Spans 1 intron

NM_001302695 · chr8

107 Specific

FWD 5' TAGCACACCGATGTCAGACC 3'

REV 5' CAGCACTGGATCCCAAAGGA 3'

Fwd Tm 61.3°C

Rev Tm 61.7°C

GC 55%

Product 108 bp

Spans 1 intron

NM_001302695 · chr8

106 Specific

FWD 5' GATCCAGTGCTGGCAGGTAT 3'

REV 5' TTGACACAGCACTCCAGCTG 3'

Fwd Tm 61.5°C

Rev Tm 62.3°C

GC 55%

Product 140 bp

Spans 1 intron