DHFR2 20 primer pairs found

Human

Show all (including non-specific)

NM_176815 · chr3

109 Specific

FWD 5' CGGATGCTGAGGCACGTAAA 3'

REV 5' AACACCTCCGAACTTGCTGG 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 93 bp

Spans 1 intron

NM_176815 · chr3

109 Specific

FWD 5' CGGATGCTGAGGCACGTAAA 3'

REV 5' ACAGCAGCGGTTAACACCTC 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 105 bp

Spans 1 intron

NM_176815 · chr3

109 Specific

FWD 5' CGGATGCTGAGGCACGTAAA 3'

REV 5' GACAGCAGCGGTTAACACCT 3'

Fwd Tm 62.3°C

Rev Tm 62.3°C

GC 55%

Product 106 bp

Spans 1 intron

NM_176815 · chr3

108 Specific

FWD 5' CGGATGCTGAGGCACGTAAA 3'

REV 5' GCGGTTAACACCTCCGAACT 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 99 bp

Spans 1 intron

NM_176815 · chr3

108 Specific

FWD 5' CGGATGCTGAGGCACGTAAA 3'

REV 5' AGCGGTTAACACCTCCGAAC 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 100 bp

Spans 1 intron

NM_176815 · chr3

108 Specific

FWD 5' CGGATGCTGAGGCACGTAAA 3'

REV 5' CAGCGGTTAACACCTCCGAA 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 101 bp

Spans 1 intron

NM_176815 · chr3

108 Specific

FWD 5' GGATTGGTCGGCGTAGCTTT 3'

REV 5' AGGAAGCCAGGCCAAGAATG 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 143 bp

Spans 1 intron

NM_176815 · chr3

108 Specific

FWD 5' GGATTGGTCGGCGTAGCTTT 3'

REV 5' CAGGAAGCCAGGCCAAGAAT 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001195643 · chr3

108 1 gene · 2 transcripts

FWD 5' TTCCGGTAGCTGGTAAAGGC 3'

REV 5' CAGCGGTTAACACCTCCGAA 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 132 bp

Spans 1 intron

NM_001195643 · chr3

108 1 gene · 2 transcripts

FWD 5' TTCCGGTAGCTGGTAAAGGC 3'

REV 5' GCGGTTAACACCTCCGAACT 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 130 bp

Spans 1 intron

NM_001195643 · chr3

108 1 gene · 2 transcripts

FWD 5' TTCCGGTAGCTGGTAAAGGC 3'

REV 5' AGCGGTTAACACCTCCGAAC 3'

Fwd Tm 61.6°C

Rev Tm 62.0°C

GC 55%

Product 131 bp

Spans 1 intron

NM_176815 · chr3

108 Specific

FWD 5' CGGATGCTGAGGCACGTAAA 3'

REV 5' TGACAGCAGCGGTTAACACC 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 107 bp

Spans 1 intron

NM_176815 · chr3

108 Specific

FWD 5' CGGATGCTGAGGCACGTAAA 3'

REV 5' TTTGGGACACAGCGACGATG 3'

Fwd Tm 62.3°C

Rev Tm 62.5°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001195643 · chr3

107 1 gene · 2 transcripts

FWD 5' CTGATACTTCCCAGGACGCG 3'

REV 5' GCGGTTAACACCTCCGAACT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 60%

Product 111 bp

Spans 1 intron

NM_001195643 · chr3

107 1 gene · 2 transcripts

FWD 5' CTGATACTTCCCAGGACGCG 3'

REV 5' AGCGGTTAACACCTCCGAAC 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 60%

Product 112 bp

Spans 1 intron

NM_001195643 · chr3

107 1 gene · 2 transcripts

FWD 5' CTGATACTTCCCAGGACGCG 3'

REV 5' CAGCGGTTAACACCTCCGAA 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 60%

Product 113 bp

Spans 1 intron

NM_001195643 · chr3

107 1 gene · 2 transcripts

FWD 5' TCCGGTAGCTGGTAAAGGCT 3'

REV 5' TGACAGCAGCGGTTAACACC 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 137 bp

Spans 1 intron

NM_001195643 · chr3

107 1 gene · 2 transcripts

FWD 5' GGCTGATACTTCCCAGGACG 3'

REV 5' CAGCGGTTAACACCTCCGAA 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 60%

Product 115 bp

Spans 1 intron

NM_001195643 · chr3

107 1 gene · 2 transcripts

FWD 5' GGCTGATACTTCCCAGGACG 3'

REV 5' GCGGTTAACACCTCCGAACT 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 60%

Product 113 bp

Spans 1 intron

NM_001195643 · chr3

107 1 gene · 2 transcripts

FWD 5' GGCTGATACTTCCCAGGACG 3'

REV 5' AGCGGTTAACACCTCCGAAC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 60%

Product 114 bp

Spans 1 intron