Diaph2 20 primer pairs found

Human

Show all (including non-specific)

NM_006729 · chrX

115 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' GAGCGTGACCTTTCCAAAGG 3'

Fwd Tm 60.8°C

Rev Tm 60.8°C

GC 55%

Product 131 bp

Spans 2 introns

NM_006729 · chrX

115 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' TAGCTCCATTGTGGCGAGAG 3'

Fwd Tm 60.8°C

Rev Tm 61.4°C

GC 55%

Product 148 bp

Spans 2 introns

NM_006729 · chrX

114 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' CGAGAGCGTGACCTTTCCAA 3'

Fwd Tm 60.8°C

Rev Tm 62.0°C

GC 55%

Product 134 bp

Spans 2 introns

NM_006729 · chrX

114 Specific

FWD 5' AGAGGGTGATGAGACTGGTG 3'

REV 5' GAGCGTGACCTTTCCAAAGG 3'

Fwd Tm 60.5°C

Rev Tm 60.8°C

GC 55%

Product 132 bp

Spans 2 introns

NM_006729 · chrX

113 Specific

FWD 5' AGAGGGTGATGAGACTGGTG 3'

REV 5' TAGCTCCATTGTGGCGAGAG 3'

Fwd Tm 60.5°C

Rev Tm 61.4°C

GC 55%

Product 149 bp

Spans 2 introns

NM_007309 · chrX

113 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' CCCTTGTTGCACAGGGATGA 3'

Fwd Tm 60.8°C

Rev Tm 62.2°C

GC 55%

Product 124 bp

Spans 2 introns

NM_006729 · chrX

113 Specific

FWD 5' AGAGGGTGATGAGACTGGTG 3'

REV 5' CGAGAGCGTGACCTTTCCAA 3'

Fwd Tm 60.5°C

Rev Tm 62.0°C

GC 55%

Product 135 bp

Spans 2 introns

NM_006729 · chrX

112 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' GATAGCTCCATTGTGGCGAG 3'

Fwd Tm 60.8°C

Rev Tm 60.2°C

GC 55%

Product 150 bp

Spans 2 introns

NM_006729 · chrX

112 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' ATAGCTCCATTGTGGCGAGA 3'

Fwd Tm 60.8°C

Rev Tm 60.7°C

GC 55%

Product 149 bp

Spans 2 introns

NM_007309 · chrX

112 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' TTAGCCCTTGTTGCACAGGG 3'

Fwd Tm 60.8°C

Rev Tm 62.5°C

GC 55%

Product 128 bp

Spans 2 introns

NM_007309 · chrX

111 Specific

FWD 5' AGAGGGTGATGAGACTGGTG 3'

REV 5' CCCTTGTTGCACAGGGATGA 3'

Fwd Tm 60.5°C

Rev Tm 62.2°C

GC 55%

Product 125 bp

Spans 2 introns

NM_006729 · chrX

111 Specific

FWD 5' AGAGGGTGATGAGACTGGTG 3'

REV 5' ATAGCTCCATTGTGGCGAGA 3'

Fwd Tm 60.5°C

Rev Tm 60.7°C

GC 55%

Product 150 bp

Spans 2 introns

NM_007309 · chrX

110 Specific

FWD 5' AGAGGGTGATGAGACTGGTG 3'

REV 5' TTAGCCCTTGTTGCACAGGG 3'

Fwd Tm 60.5°C

Rev Tm 62.5°C

GC 55%

Product 129 bp

Spans 2 introns

NM_007309 · chrX

110 Specific

FWD 5' AGAGGGTGATGAGACTGGTG 3'

REV 5' ATTAGCCCTTGTTGCACAGG 3'

Fwd Tm 60.5°C

Rev Tm 60.2°C

GC 55%

Product 130 bp

Spans 2 introns

NM_006729 · chrX

110 Specific

FWD 5' CCCTACAATCAGGTGCAGCA 3'

REV 5' CGAGAGCGTGACCTTTCCAA 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 94 bp

Spans 1 intron

NM_007309 · chrX

110 Specific

FWD 5' AGAGGGTGATGAGACTGGTG 3'

REV 5' GGATTAGCCCTTGTTGCACA 3'

Fwd Tm 60.5°C

Rev Tm 60.2°C

GC 55%

Product 132 bp

Spans 2 introns

NM_007309 · chrX

110 Specific

FWD 5' AGAGGGTGATGAGACTGGTG 3'

REV 5' TGGATTAGCCCTTGTTGCAC 3'

Fwd Tm 60.5°C

Rev Tm 60.2°C

GC 55%

Product 133 bp

Spans 2 introns

NM_007309 · chrX

110 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' ATTAGCCCTTGTTGCACAGG 3'

Fwd Tm 60.8°C

Rev Tm 60.2°C

GC 55%

Product 129 bp

Spans 2 introns

NM_007309 · chrX

110 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' GGATTAGCCCTTGTTGCACA 3'

Fwd Tm 60.8°C

Rev Tm 60.2°C

GC 55%

Product 131 bp

Spans 2 introns

NM_007309 · chrX

110 Specific

FWD 5' GAGGGTGATGAGACTGGTGT 3'

REV 5' TGGATTAGCCCTTGTTGCAC 3'

Fwd Tm 60.8°C

Rev Tm 60.2°C

GC 55%

Product 132 bp

Spans 2 introns