EAF1 10 primer pairs found

Human

Show all (including non-specific)

NM_033083 · chr3

117 Specific

FWD 5' GGGCCTCCTTCCACACTATT 3'

REV 5' AGTCATGGGTGGTGTGGATC 3'

Fwd Tm 61.2°C

Rev Tm 61.4°C

GC 55%

Product 140 bp

Spans 2 introns

NM_033083 · chr3

116 Specific

FWD 5' GGGCCTCCTTCCACACTATT 3'

REV 5' CAGTCATGGGTGGTGTGGAT 3'

Fwd Tm 61.2°C

Rev Tm 61.7°C

GC 55%

Product 141 bp

Spans 2 introns

NM_033083 · chr3

116 Specific

FWD 5' GGGCCTCCTTCCACACTATT 3'

REV 5' ACACAGTCATGGGTGGTGTG 3'

Fwd Tm 61.2°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 2 introns

NM_033083 · chr3

114 Specific

FWD 5' GGGCCTCCTTCCACACTATT 3'

REV 5' AACACAGTCATGGGTGGTGT 3'

Fwd Tm 61.2°C

Rev Tm 61.3°C

GC 55%

Product 145 bp

Spans 2 introns

NM_033083 · chr3

113 Specific

FWD 5' GGGCCTCCTTCCACACTATT 3'

REV 5' GAACACAGTCATGGGTGGTG 3'

Fwd Tm 61.2°C

Rev Tm 60.5°C

GC 55%

Product 146 bp

Spans 2 introns

NM_033083 · chr3

113 Specific

FWD 5' GGGCCTCCTTCCACACTATT 3'

REV 5' TGAACACAGTCATGGGTGGT 3'

Fwd Tm 61.2°C

Rev Tm 61.0°C

GC 55%

Product 147 bp

Spans 2 introns

NM_033083 · chr3

111 Specific

FWD 5' GGGCCTCCTTCCACACTATT 3'

REV 5' GTCATGGGTGGTGTGGATCC 3'

Fwd Tm 61.2°C

Rev Tm 62.5°C

GC 55%

Product 139 bp

Spans 2 introns

NM_033083 · chr3

111 Specific

FWD 5' CGGGCCTCCTTCCACACTAT 3'

REV 5' ACAGTCATGGGTGGTGTGGA 3'

Fwd Tm 63.5°C

Rev Tm 63.2°C

GC 60%

Product 143 bp

Spans 2 introns

NM_033083 · chr3

111 Specific

FWD 5' GCCTCCTTCCACACTATTCG 3'

REV 5' CCCTTGAACACAGTCATGGG 3'

Fwd Tm 59.8°C

Rev Tm 60.4°C

GC 55%

Product 149 bp

Spans 2 introns

NM_033083 · chr3

111 Specific

FWD 5' GCCTCCTTCCACACTATTCG 3'

REV 5' GAACACAGTCATGGGTGGTG 3'

Fwd Tm 59.8°C

Rev Tm 60.5°C

GC 55%

Product 144 bp

Spans 2 introns