No gene-specific primers found for EEF1AKMT4-ECE2. Showing all 10 primers including those that may amplify other genes. Consider validating with NCBI Primer-BLAST.

EEF1AKMT4-ECE2 10 primer pairs found

Human

Show all (including non-specific)

NM_014693 · chr3

119 2 genes · 4 transcripts

FWD 5' AAGAAGTCCTGTGTGCCGAG 3'

REV 5' GGTCCGGATTTCGCTGATCA 3'

Fwd Tm 61.8°C

Rev Tm 61.9°C

GC 55%

Product 147 bp

Spans 2 introns

NCBI BLAST

NM_014693 · chr3

116 2 genes · 4 transcripts

FWD 5' CGTCTACATCAGTGCCGACT 3'

REV 5' TCCAGATAGGCAGTGAGCAC 3'

Fwd Tm 61.2°C

Rev Tm 61.1°C

GC 55%

Product 135 bp

Spans 2 introns

NCBI BLAST

NM_014693 · chr3

116 2 genes · 4 transcripts

FWD 5' TCACCGTCTACATCAGTGCC 3'

REV 5' TCCAGATAGGCAGTGAGCAC 3'

Fwd Tm 61.3°C

Rev Tm 61.1°C

GC 55%

Product 139 bp

Spans 2 introns

NCBI BLAST

NM_014693 · chr3

113 2 genes · 4 transcripts

FWD 5' CAAGGCCCTGAACTTCGGTG 3'

REV 5' AGGGACTCATTCTGCCACCA 3'

Fwd Tm 63.1°C

Rev Tm 63.0°C

GC 60%

Product 126 bp

Spans 2 introns

NCBI BLAST

NM_014693 · chr3

113 2 genes · 4 transcripts

FWD 5' AAGAAGTCCTGTGTGCCGAG 3'

REV 5' CGGATTTCGCTGATCATCCC 3'

Fwd Tm 61.8°C

Rev Tm 60.5°C

GC 55%

Product 143 bp

Spans 2 introns

NCBI BLAST

NM_014693 · chr3

113 2 genes · 4 transcripts

FWD 5' AAGAAGTCCTGTGTGCCGAG 3'

REV 5' CCGGATTTCGCTGATCATCC 3'

Fwd Tm 61.8°C

Rev Tm 60.5°C

GC 55%

Product 144 bp

Spans 2 introns

NCBI BLAST

NM_014693 · chr3

112 2 genes · 4 transcripts

FWD 5' TGAAGGCTGCCTACAATGCT 3'

REV 5' CGTGAGAGCTCTCTGGTGTG 3'

Fwd Tm 61.5°C

Rev Tm 61.9°C

GC 50%

Product 150 bp

Spans 2 introns

NCBI BLAST

NM_014693 · chr3

112 2 genes · 4 transcripts

FWD 5' TATCCAGGTGGACCAGTCTG 3'

REV 5' TCCAGATAGGCAGTGAGCAC 3'

Fwd Tm 60.3°C

Rev Tm 61.1°C

GC 55%

Product 93 bp

Spans 2 introns

NCBI BLAST

NM_014693 · chr3

112 2 genes · 4 transcripts

FWD 5' CCTTCTATGCCCGCAACCAC 3'

REV 5' AGGGACTCATTCTGCCACCA 3'

Fwd Tm 63.3°C

Rev Tm 63.0°C

GC 60%

Product 148 bp

Spans 2 introns

NCBI BLAST

NM_014693 · chr3

112 2 genes · 4 transcripts

FWD 5' CTTCTATGCCCGCAACCACC 3'

REV 5' AGGGACTCATTCTGCCACCA 3'

Fwd Tm 63.3°C

Rev Tm 63.0°C

GC 60%

Product 147 bp

Spans 2 introns

NCBI BLAST