Efna1 20 primer pairs found

Human

Show all (including non-specific)

NM_004428 · chr1

119 1 gene · 2 transcripts

FWD 5' TTCACACCTTTCACCCTGGG 3'

REV 5' ATTGTCATGGGCCTGAGGAC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_004428 · chr1

119 1 gene · 2 transcripts

FWD 5' CCCTGGGCAAGGAGTTCAAA 3'

REV 5' CTGTGGATTGTCATGGGCCT 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 143 bp

Spans 2 introns

NM_004428 · chr1

119 Specific

FWD 5' TGAAGGTGACTGTCAGTGGC 3'

REV 5' CAGCACTGTGACCGATGCTA 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 123 bp

Spans 2 introns

NM_004428 · chr1

119 Specific

FWD 5' GAAGGTGACTGTCAGTGGCA 3'

REV 5' CAGCACTGTGACCGATGCTA 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 122 bp

Spans 2 introns

NM_004428 · chr1

119 1 gene · 2 transcripts

FWD 5' CCCTGGGCAAGGAGTTCAAA 3'

REV 5' GTGGATTGTCATGGGCCTGA 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 141 bp

Spans 2 introns

NM_004428 · chr1

119 1 gene · 2 transcripts

FWD 5' CCCTGGGCAAGGAGTTCAAA 3'

REV 5' TCCTGTGGATTGTCATGGGC 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 2 introns

NM_004428 · chr1

119 1 gene · 2 transcripts

FWD 5' CCCTGGGCAAGGAGTTCAAA 3'

REV 5' TGTGGATTGTCATGGGCCTG 3'

Fwd Tm 62.2°C

Rev Tm 62.3°C

GC 55%

Product 142 bp

Spans 2 introns

NM_004428 · chr1

118 Specific

FWD 5' TGAAGGTGACTGTCAGTGGC 3'

REV 5' TGCTATGTAGAACCCGCACC 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 108 bp

Spans 2 introns

NM_004428 · chr1

118 Specific

FWD 5' GAAGGTGACTGTCAGTGGCA 3'

REV 5' TGCTATGTAGAACCCGCACC 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 107 bp

Spans 2 introns

NM_004428 · chr1

118 Specific

FWD 5' CCCATCCACCAGCATGAAGA 3'

REV 5' TGCTATGTAGAACCCGCACC 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 142 bp

Spans 2 introns

NM_182685 · chr1

118 1 gene · 2 transcripts

FWD 5' TTCACACCTTTCACCCTGGG 3'

REV 5' TGCTATGTAGAACCCGCACC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 139 bp

Spans 2 introns

NM_182685 · chr1

118 1 gene · 2 transcripts

FWD 5' CAGCGCTTCACACCTTTCAC 3'

REV 5' TGCTATGTAGAACCCGCACC 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 145 bp

Spans 2 introns

NM_182685 · chr1

118 1 gene · 2 transcripts

FWD 5' CCCTGGGCAAGGAGTTCAAA 3'

REV 5' CAGCACTGTGACCGATGCTA 3'

Fwd Tm 62.2°C

Rev Tm 61.8°C

GC 55%

Product 141 bp

Spans 2 introns

NM_182685 · chr1

117 1 gene · 2 transcripts

FWD 5' CAGCGCTTCACACCTTTCAC 3'

REV 5' GCTATGTAGAACCCGCACCT 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 144 bp

Spans 2 introns

NM_182685 · chr1

117 1 gene · 2 transcripts

FWD 5' CAGCGCTTCACACCTTTCAC 3'

REV 5' ATGTAGAACCCGCACCTCTG 3'

Fwd Tm 61.5°C

Rev Tm 61.4°C

GC 55%

Product 141 bp

Spans 2 introns

NM_182685 · chr1

117 1 gene · 2 transcripts

FWD 5' TTCACACCTTTCACCCTGGG 3'

REV 5' GCTATGTAGAACCCGCACCT 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 138 bp

Spans 2 introns

NM_182685 · chr1

117 1 gene · 2 transcripts

FWD 5' CCCTGGGCAAGGAGTTCAAA 3'

REV 5' TGCTATGTAGAACCCGCACC 3'

Fwd Tm 62.2°C

Rev Tm 61.6°C

GC 55%

Product 126 bp

Spans 2 introns

NM_182685 · chr1

117 1 gene · 2 transcripts

FWD 5' GAAGTTCCAGCGCTTCACAC 3'

REV 5' ATGTAGAACCCGCACCTCTG 3'

Fwd Tm 61.3°C

Rev Tm 61.4°C

GC 55%

Product 148 bp

Spans 2 introns

NM_182685 · chr1

117 1 gene · 2 transcripts

FWD 5' TTCACACCTTTCACCCTGGG 3'

REV 5' ATGTAGAACCCGCACCTCTG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 135 bp

Spans 2 introns

NM_182685 · chr1

117 1 gene · 2 transcripts

FWD 5' CGCTTCACACCTTTCACCCT 3'

REV 5' TGCTATGTAGAACCCGCACC 3'

Fwd Tm 62.3°C

Rev Tm 61.6°C

GC 55%

Product 142 bp

Spans 2 introns