Eme2 10 primer pairs found

Human

Show all (including non-specific)

NM_001257370 · chr16

114 Specific

FWD 5' GATGCCTACCTGTGGTCTCG 3'

REV 5' CAGAGCTGCAGGAGTACCAG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 60%

Product 131 bp

Spans 2 introns

NM_001257370 · chr16

114 Specific

FWD 5' TGGATGCCTACCTGTGGTCT 3'

REV 5' CAGAGCTGCAGGAGTACCAG 3'

Fwd Tm 62.5°C

Rev Tm 61.8°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001257370 · chr16

114 Specific

FWD 5' CTGGATGCCTACCTGTGGTC 3'

REV 5' CAGAGCTGCAGGAGTACCAG 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 60%

Product 134 bp

Spans 2 introns

NM_001257370 · chr16

113 Specific

FWD 5' GGATGCCTACCTGTGGTCTC 3'

REV 5' CAGAGCTGCAGGAGTACCAG 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 60%

Product 132 bp

Spans 2 introns

NM_001257370 · chr16

112 Specific

FWD 5' TGGATGCCTACCTGTGGTCT 3'

REV 5' AGAGCTGCAGGAGTACCAGG 3'

Fwd Tm 62.5°C

Rev Tm 63.2°C

GC 55%

Product 132 bp

Spans 2 introns

NM_001257370 · chr16

111 Specific

FWD 5' TGGATGCCTACCTGTGGTCT 3'

REV 5' CCAGAGCTGCAGGAGTACCA 3'

Fwd Tm 62.5°C

Rev Tm 63.4°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001257370 · chr16

109 Specific

FWD 5' AAACCTGGACGTGCTACTGG 3'

REV 5' TGCTGTGCAGAAGGAGAAGG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 130 bp

Spans 1 intron

NM_001257370 · chr16

109 Specific

FWD 5' AAACCTGGACGTGCTACTGG 3'

REV 5' CCTGCTGTGCAGAAGGAGAA 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 132 bp

Spans 1 intron

NM_001257370 · chr16

109 Specific

FWD 5' AAGAGGCCCTGGTACTCCTG 3'

REV 5' AGGAGAAGGCCTGGGATTCC 3'

Fwd Tm 63.0°C

Rev Tm 63.4°C

GC 60%

Product 150 bp

Spans 2 introns

NM_001257370 · chr16

109 Specific

FWD 5' CTGGATGCCTACCTGTGGTC 3'

REV 5' AGAGCTGCAGGAGTACCAGG 3'

Fwd Tm 61.9°C

Rev Tm 63.2°C

GC 60%

Product 133 bp

Spans 2 introns