F12 10 primer pairs found

Human

Show all (including non-specific)

NM_000505 · chr5

118 Specific

FWD 5' GACCACTGCAGCAAACACAG 3'

REV 5' CGGAGAAGCTGAGGCTCAAA 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_000505 · chr5

117 Specific

FWD 5' TCGATTCCACCTTGGGAAGC 3'

REV 5' CGGCCCTTGTGGGTACATTT 3'

Fwd Tm 61.9°C

Rev Tm 62.5°C

GC 55%

Product 143 bp

Spans 2 introns

NM_000505 · chr5

117 Specific

FWD 5' TCGATTCCACCTTGGGAAGC 3'

REV 5' CATTTGTGGTACAGCTGCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 128 bp

Spans 2 introns

NM_000505 · chr5

117 Specific

FWD 5' AGTCGTTCTCACTGTCACCG 3'

REV 5' CCATCGCTGGTCCTGATCAA 3'

Fwd Tm 61.3°C

Rev Tm 61.7°C

GC 55%

Product 145 bp

Spans 2 introns

NM_000505 · chr5

116 Specific

FWD 5' AGACCACTGCAGCAAACACA 3'

REV 5' CGGAGAAGCTGAGGCTCAAA 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 50%

Product 141 bp

Spans 2 introns

NM_000505 · chr5

116 Specific

FWD 5' TCGATTCCACCTTGGGAAGC 3'

REV 5' ATTTGTGGTACAGCTGCCGG 3'

Fwd Tm 61.9°C

Rev Tm 62.7°C

GC 55%

Product 127 bp

Spans 2 introns

NM_000505 · chr5

116 Specific

FWD 5' GACCACTGCAGCAAACACAG 3'

REV 5' GCTGAGGCTCAAAGCACTTC 3'

Fwd Tm 61.5°C

Rev Tm 61.1°C

GC 55%

Product 133 bp

Spans 2 introns

NM_000505 · chr5

116 Specific

FWD 5' GACCACTGCAGCAAACACAG 3'

REV 5' GAAGCTGAGGCTCAAAGCAC 3'

Fwd Tm 61.5°C

Rev Tm 61.1°C

GC 55%

Product 136 bp

Spans 2 introns

NM_000505 · chr5

116 Specific

FWD 5' GACCACTGCAGCAAACACAG 3'

REV 5' AGCTGAGGCTCAAAGCACTT 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 134 bp

Spans 2 introns

NM_000505 · chr5

116 Specific

FWD 5' GACCACTGCAGCAAACACAG 3'

REV 5' AAGCTGAGGCTCAAAGCACT 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 135 bp

Spans 2 introns