F5 10 primer pairs found

Human

Show all (including non-specific)

NM_000130 · chr1

118 Specific

FWD 5' CCAGAGAGCAGGGATGCAAA 3'

REV 5' TTGCTAATCTGGGCTCCCAG 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 55%

Product 140 bp

Spans 2 introns

NM_000130 · chr1

118 Specific

FWD 5' CCAGAGAGCAGGGATGCAAA 3'

REV 5' CTTGCTAATCTGGGCTCCCA 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 55%

Product 141 bp

Spans 2 introns

NM_000130 · chr1

118 Specific

FWD 5' AAACCAGAGAGCAGGGATGC 3'

REV 5' TTGCTAATCTGGGCTCCCAG 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 55%

Product 143 bp

Spans 2 introns

NM_000130 · chr1

118 Specific

FWD 5' AAACCAGAGAGCAGGGATGC 3'

REV 5' CTTGCTAATCTGGGCTCCCA 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 55%

Product 144 bp

Spans 2 introns

NM_000130 · chr1

117 Specific

FWD 5' AGAGAGCAGGGATGCAAACG 3'

REV 5' TTGCTAATCTGGGCTCCCAG 3'

Fwd Tm 62.2°C

Rev Tm 61.5°C

GC 55%

Product 138 bp

Spans 2 introns

NM_000130 · chr1

117 Specific

FWD 5' AGAGAGCAGGGATGCAAACG 3'

REV 5' CTTGCTAATCTGGGCTCCCA 3'

Fwd Tm 62.2°C

Rev Tm 61.5°C

GC 55%

Product 139 bp

Spans 2 introns

NM_000130 · chr1

116 Specific

FWD 5' CCAGAGAGCAGGGATGCAAA 3'

REV 5' TCTTGCTAATCTGGGCTCCC 3'

Fwd Tm 62.0°C

Rev Tm 61.2°C

GC 55%

Product 142 bp

Spans 2 introns

NM_000130 · chr1

116 Specific

FWD 5' AAACCAGAGAGCAGGGATGC 3'

REV 5' TCTTGCTAATCTGGGCTCCC 3'

Fwd Tm 62.0°C

Rev Tm 61.2°C

GC 55%

Product 145 bp

Spans 2 introns

NM_000130 · chr1

116 Specific

FWD 5' AGAGAGCAGGGATGCAAACG 3'

REV 5' TGCTAATCTGGGCTCCCAGT 3'

Fwd Tm 62.2°C

Rev Tm 62.8°C

GC 55%

Product 137 bp

Spans 2 introns

NM_000130 · chr1

116 Specific

FWD 5' AAACCAGAGAGCAGGGATGC 3'

REV 5' TGCTAATCTGGGCTCCCAGT 3'

Fwd Tm 62.0°C

Rev Tm 62.8°C

GC 55%

Product 142 bp

Spans 2 introns