F8 20 primer pairs found

Human

Show all (including non-specific)

NM_000132 · chrX

119 Specific

FWD 5' ACAATATGGACAGTGGGCCC 3'

REV 5' CTGGGTCTTGATGCCGTGAA 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 139 bp

Spans 2 introns

NM_000132 · chrX

118 Specific

FWD 5' CATGCTGGGATGAGCACACT 3'

REV 5' TGAAGTCTGGCCAGCTTTGG 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 143 bp

Spans 2 introns

NM_000132 · chrX

118 Specific

FWD 5' TAGAGAAGGGAGTCTGGCCA 3'

REV 5' GAGCAGATGCAGCATCCCTA 3'

Fwd Tm 61.6°C

Rev Tm 61.5°C

GC 55%

Product 140 bp

Spans 2 introns

NM_000132 · chrX

118 Specific

FWD 5' TAGAGAAGGGAGTCTGGCCA 3'

REV 5' GCAGCATCCCTATCCTGCAT 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 132 bp

Spans 2 introns

NM_000132 · chrX

118 Specific

FWD 5' TAGAGAAGGGAGTCTGGCCA 3'

REV 5' ATGCAGCATCCCTATCCTGC 3'

Fwd Tm 61.6°C

Rev Tm 61.9°C

GC 55%

Product 134 bp

Spans 2 introns

NM_000132 · chrX

118 Specific

FWD 5' ACATGCTGGGATGAGCACAC 3'

REV 5' TGAAGTCTGGCCAGCTTTGG 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 144 bp

Spans 2 introns

NM_000132 · chrX

118 Specific

FWD 5' AGAGAAGGGAGTCTGGCCAA 3'

REV 5' GCAGCATCCCTATCCTGCAT 3'

Fwd Tm 62.4°C

Rev Tm 61.9°C

GC 55%

Product 131 bp

Spans 2 introns

NM_000132 · chrX

118 Specific

FWD 5' AGAGAAGGGAGTCTGGCCAA 3'

REV 5' ATGCAGCATCCCTATCCTGC 3'

Fwd Tm 62.4°C

Rev Tm 61.9°C

GC 55%

Product 133 bp

Spans 2 introns

NM_000132 · chrX

117 Specific

FWD 5' GAGAAGGGAGTCTGGCCAAG 3'

REV 5' GCAGCATCCCTATCCTGCAT 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 60%

Product 130 bp

Spans 2 introns

NM_000132 · chrX

117 Specific

FWD 5' GAGAAGGGAGTCTGGCCAAG 3'

REV 5' ATGCAGCATCCCTATCCTGC 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 60%

Product 132 bp

Spans 2 introns

NM_019863 · chrX

109 1 gene · 2 transcripts

FWD 5' CAGCACTCTTCGCATGGAGT 3'

REV 5' AAGGAGACCAGGTGGCAAAC 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 140 bp

Spans 1 intron

NM_019863 · chrX

109 1 gene · 2 transcripts

FWD 5' GTTTGCCACCTGGTCTCCTT 3'

REV 5' TCTGGAAGTCCACTTGCAGC 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 110 bp

Spans 1 intron

NM_019863 · chrX

109 Specific

FWD 5' AGACCCTGGGAAGGTCTTCT 3'

REV 5' ACTCCATGCGAAGAGTGCTG 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 137 bp

Spans 1 intron

NM_019863 · chrX

109 1 gene · 2 transcripts

FWD 5' TTTGCCACCTGGTCTCCTTC 3'

REV 5' TCTGGAAGTCCACTTGCAGC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 109 bp

Spans 1 intron

NM_019863 · chrX

109 1 gene · 2 transcripts

FWD 5' GCCACCTGGTCTCCTTCAAA 3'

REV 5' TCTGGAAGTCCACTTGCAGC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 106 bp

Spans 1 intron

NM_019863 · chrX

109 1 gene · 2 transcripts

FWD 5' CAGCACTCTTCGCATGGAGT 3'

REV 5' GAAGGAGACCAGGTGGCAAA 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 141 bp

Spans 1 intron

NM_019863 · chrX

109 1 gene · 2 transcripts

FWD 5' CAGCACTCTTCGCATGGAGT 3'

REV 5' TTTGAAGGAGACCAGGTGGC 3'

Fwd Tm 62.1°C

Rev Tm 61.9°C

GC 55%

Product 144 bp

Spans 1 intron

NM_019863 · chrX

109 1 gene · 2 transcripts

FWD 5' CAGCACTCTTCGCATGGAGT 3'

REV 5' GGAGACCAGGTGGCAAACAT 3'

Fwd Tm 62.1°C

Rev Tm 62.2°C

GC 55%

Product 138 bp

Spans 1 intron

NM_019863 · chrX

109 1 gene · 2 transcripts

FWD 5' ATGTTTGCCACCTGGTCTCC 3'

REV 5' TCTGGAAGTCCACTTGCAGC 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 112 bp

Spans 1 intron

NM_019863 · chrX

108 1 gene · 2 transcripts

FWD 5' ATGTTTGCCACCTGGTCTCC 3'

REV 5' GTCCACTTGCAGCCACTCTT 3'

Fwd Tm 62.2°C

Rev Tm 62.4°C

GC 55%

Product 105 bp

Spans 1 intron