GCLM 20 primer pairs found

Human

Show all (including non-specific)

NM_001308253 · chr1

109 Specific

FWD 5' AGCGAGGAGGAGTTTCCAGA 3'

REV 5' CAGCTGTGCAACTCCAAGGA 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 183 bp

Spans 3 introns

NM_001308253 · chr1

109 Specific

FWD 5' AGCGAGGAGGAGTTTCCAGA 3'

REV 5' TCCAGCTGTGCAACTCCAAG 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 185 bp

Spans 3 introns

NM_001308253 · chr1

109 Specific

FWD 5' AGCGAGGAGGAGTTTCCAGA 3'

REV 5' GCTGTGCAACTCCAAGGACT 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 181 bp

Spans 3 introns

NM_001308253 · chr1

109 Specific

FWD 5' AGCGAGGAGGAGTTTCCAGA 3'

REV 5' AGCTGTGCAACTCCAAGGAC 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 182 bp

Spans 3 introns

NM_001308253 · chr1

108 Specific

FWD 5' AGCGAGGAGGAGTTTCCAGA 3'

REV 5' CCAAGGACTGAACAGGCCAT 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 170 bp

Spans 2 introns

NM_001308253 · chr1

108 Specific

FWD 5' AGCGAGGAGGAGTTTCCAGA 3'

REV 5' GGACTGAACAGGCCATGTCA 3'

Fwd Tm 62.3°C

Rev Tm 61.9°C

GC 55%

Product 166 bp

Spans 2 introns

NM_001308253 · chr1

107 Specific

FWD 5' CAGCGAGGAGGAGTTTCCAG 3'

REV 5' CCAAGGACTGAACAGGCCAT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 60%

Product 171 bp

Spans 2 introns

NM_001308253 · chr1

107 Specific

FWD 5' AGCGAGGAGGAGTTTCCAGA 3'

REV 5' CAGGCCATGTCAACTGCACT 3'

Fwd Tm 62.3°C

Rev Tm 62.7°C

GC 55%

Product 158 bp

Spans 2 introns

NM_001308253 · chr1

107 Specific

FWD 5' CAGCGAGGAGGAGTTTCCAG 3'

REV 5' GGACTGAACAGGCCATGTCA 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 60%

Product 167 bp

Spans 2 introns

NM_001308253 · chr1

107 Specific

FWD 5' AGCGAGGAGGAGTTTCCAGA 3'

REV 5' AGGACTGAACAGGCCATGTC 3'

Fwd Tm 62.3°C

Rev Tm 61.6°C

GC 55%

Product 167 bp

Spans 2 introns

NM_002061 · chr1

107 1 gene · 2 transcripts

FWD 5' GCTGTGTGATGCCACCAGAT 3'

REV 5' CGTGCGCTTGAATGTCAGGA 3'

Fwd Tm 62.5°C

Rev Tm 62.8°C

GC 55%

Product 144 bp

Spans 1 intron

NM_002061 · chr1

107 1 gene · 2 transcripts

FWD 5' GCTGTGTGATGCCACCAGAT 3'

REV 5' TCGTGCGCTTGAATGTCAGG 3'

Fwd Tm 62.5°C

Rev Tm 62.8°C

GC 55%

Product 145 bp

Spans 1 intron

NM_002061 · chr1

106 Specific

FWD 5' ACCCAGATTTGGTCAGGGAG 3'

REV 5' GGCCATGTCAACTGCACTTC 3'

Fwd Tm 61.2°C

Rev Tm 61.4°C

GC 55%

Product 164 bp

Spans 2 introns

NM_002061 · chr1

106 Specific

FWD 5' ACCCAGATTTGGTCAGGGAG 3'

REV 5' GAACAGGCCATGTCAACTGC 3'

Fwd Tm 61.2°C

Rev Tm 61.4°C

GC 55%

Product 169 bp

Spans 2 introns

NM_002061 · chr1

106 Specific

FWD 5' CCCAGATTTGGTCAGGGAGT 3'

REV 5' GGCCATGTCAACTGCACTTC 3'

Fwd Tm 61.2°C

Rev Tm 61.4°C

GC 55%

Product 163 bp

Spans 2 introns

NM_002061 · chr1

106 Specific

FWD 5' CCCAGATTTGGTCAGGGAGT 3'

REV 5' GAACAGGCCATGTCAACTGC 3'

Fwd Tm 61.2°C

Rev Tm 61.4°C

GC 55%

Product 168 bp

Spans 2 introns

NM_002061 · chr1

106 Specific

FWD 5' ACCCAGATTTGGTCAGGGAG 3'

REV 5' ACTCCAAGGACTGAACAGGC 3'

Fwd Tm 61.2°C

Rev Tm 61.6°C

GC 55%

Product 181 bp

Spans 2 introns

NM_002061 · chr1

106 Specific

FWD 5' CCCAGATTTGGTCAGGGAGT 3'

REV 5' ACTCCAAGGACTGAACAGGC 3'

Fwd Tm 61.2°C

Rev Tm 61.6°C

GC 55%

Product 180 bp

Spans 2 introns

NM_002061 · chr1

106 Specific

FWD 5' ACCCAGATTTGGTCAGGGAG 3'

REV 5' AGGACTGAACAGGCCATGTC 3'

Fwd Tm 61.2°C

Rev Tm 61.6°C

GC 55%

Product 175 bp

Spans 2 introns

NM_002061 · chr1

106 Specific

FWD 5' CCCAGATTTGGTCAGGGAGT 3'

REV 5' AGGACTGAACAGGCCATGTC 3'

Fwd Tm 61.2°C

Rev Tm 61.6°C

GC 55%

Product 174 bp

Spans 2 introns