Gdf5 20 primer pairs found

Human

Show all (including non-specific)

NM_000557 · chr20

110 1 gene · 2 transcripts

FWD 5' AAAGGGCAAGATGACCGAGG 3'

REV 5' AGGGCTTCTTCCGCAAGATC 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 118 bp

Spans 1 intron

NM_000557 · chr20

110 1 gene · 2 transcripts

FWD 5' AAAGGGCAAGATGACCGAGG 3'

REV 5' GAGGGCTTCTTCCGCAAGAT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001319138 · chr20

110 1 gene · 2 transcripts

FWD 5' AAAGGGCAAGATGACCGAGG 3'

REV 5' AGGGCTTCTTCCGCAAGATC 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 118 bp

Spans 1 intron

NM_001319138 · chr20

110 1 gene · 2 transcripts

FWD 5' AAAGGGCAAGATGACCGAGG 3'

REV 5' GAGGGCTTCTTCCGCAAGAT 3'

Fwd Tm 62.0°C

Rev Tm 62.0°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001319138 · chr20

109 Specific

FWD 5' GACCGGTTTCTGAGGGTTGT 3'

REV 5' TGGATCACGAGGTCAGGAGA 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 133 bp

Spans 1 intron

NM_001319138 · chr20

108 Specific

FWD 5' ACAAGAGTCCTGTGAGCTGC 3'

REV 5' AGCAGCAGTAGCAGCAGAAG 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001319138 · chr20

108 Specific

FWD 5' ACAAGAGTCCTGTGAGCTGC 3'

REV 5' CAGCAGTAGCAGCAGAAGGA 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001319138 · chr20

108 Specific

FWD 5' CAAGAGTCCTGTGAGCTGCT 3'

REV 5' CAGCAGTAGCAGCAGAAGGA 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 146 bp

Spans 1 intron

NM_001319138 · chr20

108 Specific

FWD 5' AAGAGTCCTGTGAGCTGCTG 3'

REV 5' CAGCAGTAGCAGCAGAAGGA 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001319138 · chr20

108 Specific

FWD 5' CGAGAAGCTTCAGGCAGCAT 3'

REV 5' CGCCTGTAATCCCAGCACTT 3'

Fwd Tm 62.4°C

Rev Tm 62.2°C

GC 55%

Product 128 bp

Spans 1 intron

NM_001319138 · chr20

108 Specific

FWD 5' CGAGAAGCTTCAGGCAGCAT 3'

REV 5' CAGTGGCTCACGCCTGTAAT 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 138 bp

Spans 1 intron

NM_000557 · chr20

108 1 gene · 2 transcripts

FWD 5' AAAGGGCAAGATGACCGAGG 3'

REV 5' TTCTTCCGCAAGATCCGCAG 3'

Fwd Tm 62.0°C

Rev Tm 62.4°C

GC 55%

Product 113 bp

Spans 1 intron

NM_000557 · chr20

108 1 gene · 2 transcripts

FWD 5' AAAGGGCAAGATGACCGAGG 3'

REV 5' CTTCTTCCGCAAGATCCGCA 3'

Fwd Tm 62.0°C

Rev Tm 62.4°C

GC 55%

Product 114 bp

Spans 1 intron

NM_001319138 · chr20

108 1 gene · 2 transcripts

FWD 5' AAAGGGCAAGATGACCGAGG 3'

REV 5' TTCTTCCGCAAGATCCGCAG 3'

Fwd Tm 62.0°C

Rev Tm 62.4°C

GC 55%

Product 113 bp

Spans 1 intron

NM_000557 · chr20

107 1 gene · 2 transcripts

FWD 5' CAACAGCAGCGTGAAGTTGG 3'

REV 5' CGTACCTCTGCTTCCTGACC 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 1 intron

NM_000557 · chr20

106 1 gene · 2 transcripts

FWD 5' TCCGATGCTGACAGAAAGGG 3'

REV 5' ACACGTACCTCTGCTTCCTG 3'

Fwd Tm 61.6°C

Rev Tm 61.2°C

GC 55%

Product 130 bp

Spans 1 intron

NM_000557 · chr20

106 1 gene · 2 transcripts

FWD 5' CCGATGCTGACAGAAAGGGA 3'

REV 5' ACACGTACCTCTGCTTCCTG 3'

Fwd Tm 61.6°C

Rev Tm 61.2°C

GC 55%

Product 129 bp

Spans 1 intron

NM_000557 · chr20

106 1 gene · 2 transcripts

FWD 5' ATGCTGACAGAAAGGGAGGC 3'

REV 5' CGTACCTCTGCTTCCTGACC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 123 bp

Spans 1 intron

NM_000557 · chr20

106 1 gene · 2 transcripts

FWD 5' CAACAGCAGCGTGAAGTTGG 3'

REV 5' ACACGTACCTCTGCTTCCTG 3'

Fwd Tm 61.8°C

Rev Tm 61.2°C

GC 55%

Product 107 bp

Spans 1 intron

NM_000557 · chr20

106 1 gene · 2 transcripts

FWD 5' ATGCTGACAGAAAGGGAGGC 3'

REV 5' ACACGTACCTCTGCTTCCTG 3'

Fwd Tm 62.0°C

Rev Tm 61.2°C

GC 55%

Product 126 bp

Spans 1 intron