HCFC1 10 primer pairs found

Human

Show all (including non-specific)

NM_005334 · chrX

116 Specific

FWD 5' AATGAGAAGGGCTATGGCCC 3'

REV 5' CACTGACCATCGGCCTTAGA 3'

Fwd Tm 61.7°C

Rev Tm 61.1°C

GC 55%

Product 149 bp

Spans 2 introns

NM_005334 · chrX

116 Specific

FWD 5' AATGAGAAGGGCTATGGCCC 3'

REV 5' TCACTGACCATCGGCCTTAG 3'

Fwd Tm 61.7°C

Rev Tm 61.1°C

GC 55%

Product 150 bp

Spans 2 introns

NM_005334 · chrX

116 Specific

FWD 5' GTGGCTGCAGGAAACCAGTA 3'

REV 5' TCTCACTGACCATCGGCCTT 3'

Fwd Tm 62.1°C

Rev Tm 62.9°C

GC 55%

Product 117 bp

Spans 2 introns

NM_005334 · chrX

115 Specific

FWD 5' GGGACCTGTGGACCCTAGAT 3'

REV 5' CCAGCCACCAAACACGTACA 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 60%

Product 131 bp

Spans 2 introns

NM_005334 · chrX

115 Specific

FWD 5' GTGGCTGCAGGAAACCAGTA 3'

REV 5' CACTGACCATCGGCCTTAGA 3'

Fwd Tm 62.1°C

Rev Tm 61.1°C

GC 55%

Product 114 bp

Spans 2 introns

NM_005334 · chrX

115 Specific

FWD 5' GTGGCTGCAGGAAACCAGTA 3'

REV 5' TCACTGACCATCGGCCTTAG 3'

Fwd Tm 62.1°C

Rev Tm 61.1°C

GC 55%

Product 115 bp

Spans 2 introns

NM_005334 · chrX

115 Specific

FWD 5' GTGGCTGCAGGAAACCAGTA 3'

REV 5' CTCACTGACCATCGGCCTTA 3'

Fwd Tm 62.1°C

Rev Tm 61.1°C

GC 55%

Product 116 bp

Spans 2 introns

NM_005334 · chrX

114 Specific

FWD 5' GTGGCTGCAGGAAACCAGTA 3'

REV 5' CAGCTTCCTCTCACTGACCA 3'

Fwd Tm 62.1°C

Rev Tm 61.0°C

GC 55%

Product 125 bp

Spans 2 introns

NM_005334 · chrX

114 Specific

FWD 5' ATTGACACCCTGACGTGGAA 3'

REV 5' GTGGCCACTTTGACGTCATC 3'

Fwd Tm 61.0°C

Rev Tm 61.0°C

GC 50%

Product 146 bp

Spans 2 introns

NM_005334 · chrX

114 Specific

FWD 5' TTGACACCCTGACGTGGAAT 3'

REV 5' GTGGCCACTTTGACGTCATC 3'

Fwd Tm 61.0°C

Rev Tm 61.0°C

GC 50%

Product 145 bp

Spans 2 introns