HSD3B1 20 primer pairs found

Human

Show all (including non-specific)

NM_001328615 · chr1

109 1 gene · 2 transcripts

FWD 5' AGGAGGGTTTCTGGGACAGA 3'

REV 5' CCTTCCAGCACTGTCAGCTT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001328615 · chr1

109 1 gene · 2 transcripts

FWD 5' AGGGTTTCTGGGACAGAGGA 3'

REV 5' CCTTCCAGCACTGTCAGCTT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 147 bp

Spans 1 intron

NM_000862 · chr1

109 1 gene · 2 transcripts

FWD 5' AGGAGGGTTTCTGGGACAGA 3'

REV 5' CCTTCCAGCACTGTCAGCTT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 150 bp

Spans 1 intron

NM_000862 · chr1

109 1 gene · 2 transcripts

FWD 5' AGGGTTTCTGGGACAGAGGA 3'

REV 5' CCTTCCAGCACTGTCAGCTT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 147 bp

Spans 1 intron

NM_001328615 · chr1

109 1 gene · 2 transcripts

FWD 5' AGGAGGGTTTCTGGGACAGA 3'

REV 5' CAGCACTGTCAGCTTGGTCT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 1 intron

NM_001328615 · chr1

109 1 gene · 2 transcripts

FWD 5' AGGGTTTCTGGGACAGAGGA 3'

REV 5' CAGCACTGTCAGCTTGGTCT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 1 intron

NM_000862 · chr1

109 1 gene · 2 transcripts

FWD 5' AGGAGGGTTTCTGGGACAGA 3'

REV 5' CAGCACTGTCAGCTTGGTCT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 145 bp

Spans 1 intron

NM_000862 · chr1

109 1 gene · 2 transcripts

FWD 5' AGGGTTTCTGGGACAGAGGA 3'

REV 5' CAGCACTGTCAGCTTGGTCT 3'

Fwd Tm 62.1°C

Rev Tm 62.0°C

GC 55%

Product 142 bp

Spans 1 intron

NM_001328615 · chr1

109 1 gene · 2 transcripts

FWD 5' ACAGAGGATCATCCGCCTCT 3'

REV 5' CCTTCCAGCACTGTCAGCTT 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 135 bp

Spans 1 intron

NM_000862 · chr1

109 1 gene · 2 transcripts

FWD 5' ACAGAGGATCATCCGCCTCT 3'

REV 5' CCTTCCAGCACTGTCAGCTT 3'

Fwd Tm 62.2°C

Rev Tm 62.1°C

GC 55%

Product 135 bp

Spans 1 intron

NM_001328615 · chr1

109 1 gene · 2 transcripts

FWD 5' ACAGAGGATCATCCGCCTCT 3'

REV 5' CAGCACTGTCAGCTTGGTCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 130 bp

Spans 1 intron

NM_000862 · chr1

109 1 gene · 2 transcripts

FWD 5' ACAGAGGATCATCCGCCTCT 3'

REV 5' CAGCACTGTCAGCTTGGTCT 3'

Fwd Tm 62.2°C

Rev Tm 62.0°C

GC 55%

Product 130 bp

Spans 1 intron

NM_000862 · chr1

109 Specific

FWD 5' TAATGCCCTCTCCAGGGTCA 3'

REV 5' TCTGTCCCAGAAACCCTCCT 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 142 bp

Spans 1 intron

NM_000862 · chr1

109 Specific

FWD 5' TAATGCCCTCTCCAGGGTCA 3'

REV 5' TCCTCTGTCCCAGAAACCCT 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 145 bp

Spans 1 intron

NM_000862 · chr1

109 Specific

FWD 5' TAATGCCCTCTCCAGGGTCA 3'

REV 5' TGGCCATCCAAAGTAGCAGG 3'

Fwd Tm 62.3°C

Rev Tm 62.0°C

GC 55%

Product 91 bp

Spans 1 intron

NM_000862 · chr1

109 Specific

FWD 5' ACTAATGCCCTCTCCAGGGT 3'

REV 5' TCTGTCCCAGAAACCCTCCT 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 144 bp

Spans 1 intron

NM_001328615 · chr1

108 1 gene · 2 transcripts

FWD 5' CATCATCCACACCGCCTGTA 3'

REV 5' CTGGCACACTAGCTTGGACA 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 122 bp

Spans 1 intron

NM_001328615 · chr1

108 1 gene · 2 transcripts

FWD 5' CATCCACACCGCCTGTATCA 3'

REV 5' CTGGCACACTAGCTTGGACA 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 119 bp

Spans 1 intron

NM_001328615 · chr1

108 1 gene · 2 transcripts

FWD 5' CATCATCCACACCGCCTGTA 3'

REV 5' ACACTAGCTTGGACACAGGC 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 117 bp

Spans 1 intron

NM_001328615 · chr1

108 1 gene · 2 transcripts

FWD 5' CATCATCCACACCGCCTGTA 3'

REV 5' ACTGGCACACTAGCTTGGAC 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 123 bp

Spans 1 intron