Hira 10 primer pairs found

Human

Show all (including non-specific)

NM_003325 · chr22

119 Specific

FWD 5' GCATGTGTGAACTGTGTGCG 3'

REV 5' TGGCAAGCTTACCACTGGAG 3'

Fwd Tm 62.0°C

Rev Tm 61.9°C

GC 55%

Product 139 bp

Spans 2 introns

NM_003325 · chr22

119 Specific

FWD 5' ATGAAGCTCCTGAAGCCGAC 3'

REV 5' AACCTTCCCAGAATCCTGCC 3'

Fwd Tm 61.9°C

Rev Tm 61.8°C

GC 55%

Product 120 bp

Spans 2 introns

NM_003325 · chr22

119 Specific

FWD 5' GGTCAACCACAATGGCAAGC 3'

REV 5' AACCTTCCCAGAATCCTGCC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 97 bp

Spans 2 introns

NM_003325 · chr22

119 Specific

FWD 5' GTCAACCACAATGGCAAGCC 3'

REV 5' AACCTTCCCAGAATCCTGCC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 96 bp

Spans 2 introns

NM_003325 · chr22

119 Specific

FWD 5' GCATGTGTGAACTGTGTGCG 3'

REV 5' TTACCACTGGAGCCGAACAC 3'

Fwd Tm 62.0°C

Rev Tm 61.8°C

GC 55%

Product 131 bp

Spans 2 introns

NM_003325 · chr22

118 Specific

FWD 5' CGCTTTCTGTCTGGCTCACA 3'

REV 5' AGCATACCAAGATGCCCAGC 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 117 bp

Spans 2 introns

NM_003325 · chr22

118 Specific

FWD 5' CGACCTGGGTCAACCACAAT 3'

REV 5' AACCTTCCCAGAATCCTGCC 3'

Fwd Tm 62.1°C

Rev Tm 61.8°C

GC 55%

Product 104 bp

Spans 2 introns

NM_003325 · chr22

118 Specific

FWD 5' CGCTTTCTGTCTGGCTCACA 3'

REV 5' AAGATGCCCAGCCCATTCAG 3'

Fwd Tm 62.3°C

Rev Tm 62.4°C

GC 55%

Product 109 bp

Spans 2 introns

NM_003325 · chr22

118 Specific

FWD 5' GGTCAACCACAATGGCAAGC 3'

REV 5' TCCTGGAGGACTGGAGACAT 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 129 bp

Spans 2 introns

NM_003325 · chr22

118 Specific

FWD 5' GTCAACCACAATGGCAAGCC 3'

REV 5' TCCTGGAGGACTGGAGACAT 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 55%

Product 128 bp

Spans 2 introns