IGFALS 20 primer pairs found

Human

Show all (including non-specific)

NM_001146006 · chr16

99 Specific

FWD 5' CAGGATGGCCCTGAGGAAAG 3'

REV 5' CCCGAGTGAGCCTGATACCA 3'

Fwd Tm 62.3°C

Rev Tm 63.2°C

GC 60%

Product 90 bp

Spans 1 intron

NM_001146006 · chr16

98 Specific

FWD 5' CAGGATGGCCCTGAGGAAAG 3'

REV 5' CTGCCCGAGTGAGCCTGATA 3'

Fwd Tm 62.3°C

Rev Tm 63.5°C

GC 60%

Product 93 bp

Spans 1 intron

NM_001146006 · chr16

97 Specific

FWD 5' CAGCAGACGTACCCTCCCTC 3'

REV 5' TGATACCAGCACAGCCGGAA 3'

Fwd Tm 63.9°C

Rev Tm 63.5°C

GC 65%

Product 126 bp

Spans 1 intron

NM_004970 · chr16

97 Specific

FWD 5' GATGGCCCTGAGGAAAGGAG 3'

REV 5' GCAGAAGACGCTGAGCTCAT 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 60%

Product 181 bp

Spans 1 intron

NM_001146006 · chr16

97 Specific

FWD 5' CAGGATGGCCCTGAGGAAAG 3'

REV 5' TGCCCGAGTGAGCCTGATAC 3'

Fwd Tm 62.3°C

Rev Tm 63.7°C

GC 60%

Product 92 bp

Spans 1 intron

NM_004970 · chr16

97 Specific

FWD 5' ATGGCCCTGAGGAAAGGAGG 3'

REV 5' ATCGTAGCTGCAGACACAGG 3'

Fwd Tm 63.7°C

Rev Tm 61.5°C

GC 60%

Product 150 bp

Spans 1 intron

NM_001146006 · chr16

97 Specific

FWD 5' CAGCAGACGTACCCTCCCTC 3'

REV 5' TGTGAACTGAGGCTCGAGGT 3'

Fwd Tm 63.9°C

Rev Tm 62.8°C

GC 65%

Product 94 bp

Spans 1 intron

NM_001146006 · chr16

97 Specific

FWD 5' CAGCAGACGTACCCTCCCTC 3'

REV 5' AGGTGTGAACTGAGGCTCGA 3'

Fwd Tm 63.9°C

Rev Tm 62.8°C

GC 65%

Product 97 bp

Spans 1 intron

NM_001146006 · chr16

96 Specific

FWD 5' CAGCAGACGTACCCTCCCTC 3'

REV 5' TGAACTGAGGCTCGAGGTCA 3'

Fwd Tm 63.9°C

Rev Tm 62.5°C

GC 65%

Product 92 bp

Spans 1 intron

NM_004970 · chr16

96 1 gene · 2 transcripts

FWD 5' CAGGATGGCCCTGAGGAAAG 3'

REV 5' GCAGAAGACGCTGAGCTCAT 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 60%

Product 184 bp

Spans 1 intron

NM_004970 · chr16

96 Specific

FWD 5' GATGGCCCTGAGGAAAGGAG 3'

REV 5' CGTCATCGTAGCTGCAGACA 3'

Fwd Tm 62.0°C

Rev Tm 61.7°C

GC 60%

Product 155 bp

Spans 1 intron

NM_001146006 · chr16

96 Specific

FWD 5' AGGATGGCCCTGAGGAAAGC 3'

REV 5' TGCCCGAGTGAGCCTGATAC 3'

Fwd Tm 64.2°C

Rev Tm 63.7°C

GC 60%

Product 91 bp

Spans 1 intron

NM_001146006 · chr16

95 Specific

FWD 5' ACAGCAGACGTACCCTCCCT 3'

REV 5' TGATACCAGCACAGCCGGAA 3'

Fwd Tm 64.7°C

Rev Tm 63.5°C

GC 60%

Product 127 bp

Spans 1 intron

NM_001146006 · chr16

95 Specific

FWD 5' AGGATGGCCCTGAGGAAAGC 3'

REV 5' CTGCCCGAGTGAGCCTGATA 3'

Fwd Tm 64.2°C

Rev Tm 63.5°C

GC 60%

Product 92 bp

Spans 1 intron

NM_004970 · chr16

95 Specific

FWD 5' GATGGCCCTGAGGAAAGGAG 3'

REV 5' TTCCTGGAGCTGCAGAAGAC 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 60%

Product 192 bp

Spans 1 intron

NM_004970 · chr16

95 Specific

FWD 5' GATGGCCCTGAGGAAAGGAG 3'

REV 5' GTTCCTGGAGCTGCAGAAGA 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 60%

Product 193 bp

Spans 1 intron

NM_004970 · chr16

95 Specific

FWD 5' ATGGCCCTGAGGAAAGGAGG 3'

REV 5' TCGTAGCTGCAGACACAGGC 3'

Fwd Tm 63.7°C

Rev Tm 64.3°C

GC 60%

Product 149 bp

Spans 1 intron

NM_004970 · chr16

95 Specific

FWD 5' GATGGCCCTGAGGAAAGGAG 3'

REV 5' ATCGTAGCTGCAGACACAGG 3'

Fwd Tm 62.0°C

Rev Tm 61.5°C

GC 60%

Product 151 bp

Spans 1 intron

NM_004970 · chr16

95 Specific

FWD 5' GATGGCCCTGAGGAAAGGAG 3'

REV 5' TCGTCATCGTAGCTGCAGAC 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 60%

Product 156 bp

Spans 1 intron

NM_004970 · chr16

95 Specific

FWD 5' GATGGCCCTGAGGAAAGGAG 3'

REV 5' GTCGTCATCGTAGCTGCAGA 3'

Fwd Tm 62.0°C

Rev Tm 61.4°C

GC 60%

Product 157 bp

Spans 1 intron