ISCA2 20 primer pairs found

Human

Show all (including non-specific)

NM_194279 · chr14

110 Specific

FWD 5' GTGGATGCTCCGGATTCCAA 3'

REV 5' AGTCAACCACCACTCTTGCC 3'

Fwd Tm 62.0°C

Rev Tm 62.1°C

GC 55%

Product 99 bp

Spans 1 intron

NM_194279 · chr14

110 Specific

FWD 5' CTCACAGACAGTTGCGTCCA 3'

REV 5' TTGGAATCCGGAGCATCCAC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 96 bp

Spans 1 intron

NM_194279 · chr14

110 Specific

FWD 5' TCACAGACAGTTGCGTCCAG 3'

REV 5' TTGGAATCCGGAGCATCCAC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 95 bp

Spans 1 intron

NM_194279 · chr14

110 Specific

FWD 5' CACAGACAGTTGCGTCCAGA 3'

REV 5' TTGGAATCCGGAGCATCCAC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 94 bp

Spans 1 intron

NM_194279 · chr14

109 1 gene · 2 transcripts

FWD 5' CTCACAGACAGTTGCGTCCA 3'

REV 5' AGTCAACCACCACTCTTGCC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 175 bp

Spans 2 introns

NM_194279 · chr14

109 1 gene · 2 transcripts

FWD 5' TCACAGACAGTTGCGTCCAG 3'

REV 5' AGTCAACCACCACTCTTGCC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 174 bp

Spans 2 introns

NM_194279 · chr14

109 Specific

FWD 5' CACAGACAGTTGCGTCCAGA 3'

REV 5' AGTCAACCACCACTCTTGCC 3'

Fwd Tm 61.9°C

Rev Tm 62.1°C

GC 55%

Product 173 bp

Spans 2 introns

NM_194279 · chr14

109 Specific

FWD 5' ATCCGCCTCACAGACAGTTG 3'

REV 5' TTGGAATCCGGAGCATCCAC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 102 bp

Spans 1 intron

NM_194279 · chr14

109 Specific

FWD 5' ATTCCTCAGGCTGCAAGTGG 3'

REV 5' AGTCAACCACCACTCTTGCC 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 55%

Product 125 bp

Spans 1 intron

NM_194279 · chr14

109 1 gene · 2 transcripts

FWD 5' ATCCGCCTCACAGACAGTTG 3'

REV 5' AGTCAACCACCACTCTTGCC 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 55%

Product 181 bp

Spans 2 introns

NM_001272007 · chr14

108 Specific

FWD 5' GACAGTTGCGTCCAGGGTAT 3'

REV 5' TCTTGGCTGAAGTCCACCTG 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 102 bp

Spans 1 intron

NM_001272007 · chr14

108 1 gene · 2 transcripts

FWD 5' GATCCGCCTCACAGACAGTT 3'

REV 5' TCTTGGCTGAAGTCCACCTG 3'

Fwd Tm 61.5°C

Rev Tm 61.6°C

GC 55%

Product 115 bp

Spans 1 intron

NM_001272007 · chr14

108 1 gene · 2 transcripts

FWD 5' ATCCGCCTCACAGACAGTTG 3'

REV 5' TCTTGGCTGAAGTCCACCTG 3'

Fwd Tm 61.8°C

Rev Tm 61.6°C

GC 55%

Product 114 bp

Spans 1 intron

NM_001272007 · chr14

108 1 gene · 2 transcripts

FWD 5' CTCACAGACAGTTGCGTCCA 3'

REV 5' TCTTGGCTGAAGTCCACCTG 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 108 bp

Spans 1 intron

NM_001272007 · chr14

108 1 gene · 2 transcripts

FWD 5' TCACAGACAGTTGCGTCCAG 3'

REV 5' TCTTGGCTGAAGTCCACCTG 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 107 bp

Spans 1 intron

NM_001272007 · chr14

107 1 gene · 2 transcripts

FWD 5' AGATCCGCCTCACAGACAGT 3'

REV 5' CCTTTCACGAAGGCCAAGCT 3'

Fwd Tm 62.6°C

Rev Tm 62.7°C

GC 55%

Product 92 bp

Spans 1 intron

NM_001272007 · chr14

106 1 gene · 2 transcripts

FWD 5' ATCCGCCTCACAGACAGTTG 3'

REV 5' CCTTTCACGAAGGCCAAGCT 3'

Fwd Tm 61.8°C

Rev Tm 62.7°C

GC 55%

Product 90 bp

Spans 1 intron

NM_001272007 · chr14

105 1 gene · 2 transcripts

FWD 5' CAGATCCGCCTCACAGACAG 3'

REV 5' TCTTGGCTGAAGTCCACCTG 3'

Fwd Tm 62.0°C

Rev Tm 61.6°C

GC 60%

Product 117 bp

Spans 1 intron

NM_001272007 · chr14

105 1 gene · 2 transcripts

FWD 5' GATCCGCCTCACAGACAGTT 3'

REV 5' GCTTCGGATCAGTTCTTGGC 3'

Fwd Tm 61.5°C

Rev Tm 60.9°C

GC 55%

Product 128 bp

Spans 1 intron

NM_001272007 · chr14

105 Specific

FWD 5' GACAGTTGCGTCCAGGGTAT 3'

REV 5' GCTTCGGATCAGTTCTTGGC 3'

Fwd Tm 61.6°C

Rev Tm 60.9°C

GC 55%

Product 115 bp

Spans 1 intron