No gene-specific primers found for ISY1. Showing all 20 primers including those that may amplify other genes. Consider validating with NCBI Primer-BLAST.

ISY1 20 primer pairs found

Human

Show all (including non-specific)

NM_001199469 · chr3

118 2 genes · 3 transcripts

FWD 5' TTCTGGGCCTGTGGAGTTTC 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 137 bp

Spans 2 introns

NCBI BLAST

NM_001199469 · chr3

118 2 genes · 3 transcripts

FWD 5' GGCCTGTGGAGTTTCTTGGA 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 132 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

118 2 genes · 3 transcripts

FWD 5' TTCTGGGCCTGTGGAGTTTC 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 137 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

118 2 genes · 3 transcripts

FWD 5' GGCCTGTGGAGTTTCTTGGA 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 132 bp

Spans 2 introns

NCBI BLAST

NM_001199469 · chr3

118 2 genes · 3 transcripts

FWD 5' CTTCTGGGCCTGTGGAGTTT 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 138 bp

Spans 2 introns

NCBI BLAST

NM_001199469 · chr3

118 2 genes · 3 transcripts

FWD 5' CTGGGCCTGTGGAGTTTCTT 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 135 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

118 2 genes · 3 transcripts

FWD 5' CTTCTGGGCCTGTGGAGTTT 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 138 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

118 2 genes · 3 transcripts

FWD 5' CTGGGCCTGTGGAGTTTCTT 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 61.9°C

Rev Tm 61.6°C

GC 55%

Product 135 bp

Spans 2 introns

NCBI BLAST

NM_001199469 · chr3

117 2 genes · 3 transcripts

FWD 5' TGGGCCTGTGGAGTTTCTTG 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 134 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

117 2 genes · 3 transcripts

FWD 5' TGGGCCTGTGGAGTTTCTTG 3'

REV 5' TTTCCCTCTTCCAGCTGAGC 3'

Fwd Tm 62.1°C

Rev Tm 61.6°C

GC 55%

Product 134 bp

Spans 2 introns

NCBI BLAST

NM_001199469 · chr3

117 2 genes · 3 transcripts

FWD 5' TTCTGGGCCTGTGGAGTTTC 3'

REV 5' GCGAAATCTTGCTAAGGCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 114 bp

Spans 2 introns

NCBI BLAST

NM_001199469 · chr3

117 2 genes · 3 transcripts

FWD 5' GGCCTGTGGAGTTTCTTGGA 3'

REV 5' GCGAAATCTTGCTAAGGCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 109 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

117 2 genes · 3 transcripts

FWD 5' TTCTGGGCCTGTGGAGTTTC 3'

REV 5' GCGAAATCTTGCTAAGGCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 114 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

117 2 genes · 3 transcripts

FWD 5' GGCCTGTGGAGTTTCTTGGA 3'

REV 5' GCGAAATCTTGCTAAGGCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 109 bp

Spans 2 introns

NCBI BLAST

NM_001199469 · chr3

117 2 genes · 3 transcripts

FWD 5' CTTCTGGGCCTGTGGAGTTT 3'

REV 5' GCGAAATCTTGCTAAGGCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 115 bp

Spans 2 introns

NCBI BLAST

NM_001199469 · chr3

117 2 genes · 3 transcripts

FWD 5' CTGGGCCTGTGGAGTTTCTT 3'

REV 5' GCGAAATCTTGCTAAGGCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 112 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

117 2 genes · 3 transcripts

FWD 5' CTTCTGGGCCTGTGGAGTTT 3'

REV 5' GCGAAATCTTGCTAAGGCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 115 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

117 2 genes · 3 transcripts

FWD 5' CTGGGCCTGTGGAGTTTCTT 3'

REV 5' GCGAAATCTTGCTAAGGCCG 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 112 bp

Spans 2 introns

NCBI BLAST

NM_001199469 · chr3

117 2 genes · 3 transcripts

FWD 5' TATGCAGTCACCGAGGAGGA 3'

REV 5' TTCTTCCTTCGCACCAGTGC 3'

Fwd Tm 62.1°C

Rev Tm 62.6°C

GC 55%

Product 137 bp

Spans 2 introns

NCBI BLAST

NM_020701 · chr3

117 2 genes · 3 transcripts

FWD 5' TATGCAGTCACCGAGGAGGA 3'

REV 5' TTCTTCCTTCGCACCAGTGC 3'

Fwd Tm 62.1°C

Rev Tm 62.6°C

GC 55%

Product 137 bp

Spans 2 introns

NCBI BLAST