Il4 30 primer pairs found

Human

Show all (including non-specific)

NM_000589 · chr5

119 1 gene · 2 transcripts

FWD 5' AGCAGAAGACTCTGTGCACC 3'

REV 5' GCAGCGAGTGTCCTTCTCAT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001354990 · chr5

119 Specific

FWD 5' TGAACAGCCTCACAGAGCAG 3'

REV 5' AGATGGTGCCTGTGTGTCTG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 114 bp

Spans 2 introns

NM_001354990 · chr5

119 Specific

FWD 5' TGAACAGCCTCACAGAGCAG 3'

REV 5' CAGATGGTGCCTGTGTGTCT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 115 bp

Spans 2 introns

NM_001354990 · chr5

119 Specific

FWD 5' AGCAGAAGACTCTGTGCACC 3'

REV 5' AGATGGTGCCTGTGTGTCTG 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 99 bp

Spans 2 introns

NM_001354990 · chr5

119 Specific

FWD 5' AGCAGAAGACTCTGTGCACC 3'

REV 5' CAGATGGTGCCTGTGTGTCT 3'

Fwd Tm 61.8°C

Rev Tm 61.8°C

GC 55%

Product 100 bp

Spans 2 introns

NM_000589 · chr5

119 1 gene · 3 transcripts

FWD 5' TGAACAGCCTCACAGAGCAG 3'

REV 5' ATGGTGGCTGTAGAACTGCC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 137 bp

Spans 2 introns

NM_000589 · chr5

119 1 gene · 2 transcripts

FWD 5' AGCAGAAGACTCTGTGCACC 3'

REV 5' ATGGTGGCTGTAGAACTGCC 3'

Fwd Tm 61.8°C

Rev Tm 62.0°C

GC 55%

Product 122 bp

Spans 2 introns

NM_000589 · chr5

119 1 gene · 2 transcripts

FWD 5' GACTCTGTGCACCGAGTTGA 3'

REV 5' GTCCTTCTCATGGTGGCTGT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 124 bp

Spans 2 introns

NM_000589 · chr5

119 1 gene · 2 transcripts

FWD 5' GACTCTGTGCACCGAGTTGA 3'

REV 5' GTGTCCTTCTCATGGTGGCT 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 126 bp

Spans 2 introns

NM_000589 · chr5

119 1 gene · 2 transcripts

FWD 5' GACTCTGTGCACCGAGTTGA 3'

REV 5' AGTGTCCTTCTCATGGTGGC 3'

Fwd Tm 61.6°C

Rev Tm 61.6°C

GC 55%

Product 127 bp

Spans 2 introns

NM_000589 · chr5

119 1 gene · 2 transcripts

FWD 5' AGACTCTGTGCACCGAGTTG 3'

REV 5' GTCCTTCTCATGGTGGCTGT 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 125 bp

Spans 2 introns

NM_000589 · chr5

119 1 gene · 2 transcripts

FWD 5' AGACTCTGTGCACCGAGTTG 3'

REV 5' GTGTCCTTCTCATGGTGGCT 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 127 bp

Spans 2 introns

NM_000589 · chr5

119 1 gene · 2 transcripts

FWD 5' AGACTCTGTGCACCGAGTTG 3'

REV 5' AGTGTCCTTCTCATGGTGGC 3'

Fwd Tm 61.7°C

Rev Tm 61.6°C

GC 55%

Product 128 bp

Spans 2 introns

NM_000589 · chr5

119 1 gene · 2 transcripts

FWD 5' AGACTCTGTGCACCGAGTTG 3'

REV 5' GCAGCGAGTGTCCTTCTCAT 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 134 bp

Spans 2 introns

NM_001354990 · chr5

119 Specific

FWD 5' AGACTCTGTGCACCGAGTTG 3'

REV 5' AGATGGTGCCTGTGTGTCTG 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 93 bp

Spans 2 introns

NM_001354990 · chr5

119 Specific

FWD 5' AGACTCTGTGCACCGAGTTG 3'

REV 5' CAGATGGTGCCTGTGTGTCT 3'

Fwd Tm 61.7°C

Rev Tm 61.8°C

GC 55%

Product 94 bp

Spans 2 introns

NM_001354990 · chr5

118 Specific

FWD 5' GACTCTGTGCACCGAGTTGA 3'

REV 5' AGATGGTGCCTGTGTGTCTG 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 92 bp

Spans 2 introns

NM_001354990 · chr5

118 Specific

FWD 5' GACTCTGTGCACCGAGTTGA 3'

REV 5' CAGATGGTGCCTGTGTGTCT 3'

Fwd Tm 61.6°C

Rev Tm 61.8°C

GC 55%

Product 93 bp

Spans 2 introns

NM_001354990 · chr5

118 Specific

FWD 5' ACAGCCTCACAGAGCAGAAG 3'

REV 5' AGATGGTGCCTGTGTGTCTG 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 111 bp

Spans 2 introns

NM_001354990 · chr5

118 Specific

FWD 5' ACAGCCTCACAGAGCAGAAG 3'

REV 5' CAGATGGTGCCTGTGTGTCT 3'

Fwd Tm 61.5°C

Rev Tm 61.8°C

GC 55%

Product 112 bp

Spans 2 introns

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