Isca1 10 primer pairs found

Human

Show all (including non-specific)

NM_030940 · chr9

120 Specific

FWD 5' TGAGCAAGAGGAAGCTGCAG 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 128 bp

Spans 2 introns

NM_030940 · chr9

119 Specific

FWD 5' GCTGTGAGCAAGAGGAAGCT 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 62.1°C

Rev Tm 62.1°C

GC 55%

Product 132 bp

Spans 2 introns

NM_030940 · chr9

118 Specific

FWD 5' TGTGAGCAAGAGGAAGCTGC 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 130 bp

Spans 2 introns

NM_030940 · chr9

118 Specific

FWD 5' GTGAGCAAGAGGAAGCTGCA 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 62.4°C

Rev Tm 62.1°C

GC 55%

Product 129 bp

Spans 2 introns

NM_030940 · chr9

116 Specific

FWD 5' CCTCACCCTGACACCTTCAG 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 61.8°C

Rev Tm 62.1°C

GC 60%

Product 94 bp

Spans 2 introns

NM_030940 · chr9

116 Specific

FWD 5' CTCACCCTGACACCTTCAGC 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 62.3°C

Rev Tm 62.1°C

GC 60%

Product 93 bp

Spans 2 introns

NM_030940 · chr9

115 Specific

FWD 5' CACCCTGACACCTTCAGCAG 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 62.6°C

Rev Tm 62.1°C

GC 60%

Product 91 bp

Spans 2 introns

NM_030940 · chr9

115 Specific

FWD 5' TCACCCTGACACCTTCAGCA 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 63.1°C

Rev Tm 62.1°C

GC 55%

Product 92 bp

Spans 2 introns

NM_030940 · chr9

114 Specific

FWD 5' ACCCTGACACCTTCAGCAGT 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 63.1°C

Rev Tm 62.1°C

GC 55%

Product 90 bp

Spans 2 introns

NM_030940 · chr9

114 Specific

FWD 5' GGGCTGTGAGCAAGAGGAAG 3'

REV 5' CCTGGTTCGGACACCAACTT 3'

Fwd Tm 62.7°C

Rev Tm 62.1°C

GC 60%

Product 134 bp

Spans 2 introns