LOC100002392 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001361396 · chrUn_KN149936v1

111 Specific

FWD 5' GTCTTCCATGTGTGAAGGCC 3'

REV 5' GTGTTTGCGTGGATGTCTGT 3'

Fwd Tm 60.6°C

Rev Tm 60.5°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001361396 · chrUn_KN149936v1

111 Specific

FWD 5' GTCTTCCATGTGTGAAGGCC 3'

REV 5' GTCAGTGTTTGCGTGGATGT 3'

Fwd Tm 60.6°C

Rev Tm 60.5°C

GC 55%

Product 145 bp

Spans 2 introns

NM_001361396 · chrUn_KN149936v1

111 Specific

FWD 5' GTCTTCCATGTGTGAAGGCC 3'

REV 5' TGTCAGTGTTTGCGTGGATG 3'

Fwd Tm 60.6°C

Rev Tm 60.5°C

GC 55%

Product 146 bp

Spans 2 introns

NM_001361396 · chrUn_KN149936v1

110 Specific

FWD 5' GTCTTCCATGTGTGAAGGCC 3'

REV 5' AGTGTTTGCGTGGATGTCTG 3'

Fwd Tm 60.6°C

Rev Tm 60.3°C

GC 55%

Product 142 bp

Spans 2 introns

NM_001361396 · chrUn_KN149936v1

110 Specific

FWD 5' GTCTTCCATGTGTGAAGGCC 3'

REV 5' CAGTGTTTGCGTGGATGTCT 3'

Fwd Tm 60.6°C

Rev Tm 60.3°C

GC 55%

Product 143 bp

Spans 2 introns

NM_001361396 · chrUn_KN149936v1

110 Specific

FWD 5' GTCTTCCATGTGTGAAGGCC 3'

REV 5' AGATGTCAGTGTTTGCGTGG 3'

Fwd Tm 60.6°C

Rev Tm 60.3°C

GC 55%

Product 149 bp

Spans 2 introns

NM_001361396 · chrUn_KN149936v1

110 Specific

FWD 5' GTCTTCCATGTGTGAAGGCC 3'

REV 5' TCAGTGTTTGCGTGGATGTC 3'

Fwd Tm 60.6°C

Rev Tm 60.2°C

GC 55%

Product 144 bp

Spans 2 introns

NM_001361396 · chrUn_KN149936v1

110 Specific

FWD 5' GTCTTCCATGTGTGAAGGCC 3'

REV 5' GATGTCAGTGTTTGCGTGGA 3'

Fwd Tm 60.6°C

Rev Tm 60.2°C

GC 55%

Product 148 bp

Spans 2 introns

NM_001361396 · chrUn_KN149936v1

108 Specific

FWD 5' TCTTCCATGTGTGAAGGCCA 3'

REV 5' GTGTTTGCGTGGATGTCTGT 3'

Fwd Tm 61.1°C

Rev Tm 60.5°C

GC 50%

Product 140 bp

Spans 2 introns

NM_001361396 · chrUn_KN149936v1

108 Specific

FWD 5' TCTTCCATGTGTGAAGGCCA 3'

REV 5' GTCAGTGTTTGCGTGGATGT 3'

Fwd Tm 61.1°C

Rev Tm 60.5°C

GC 50%

Product 144 bp

Spans 2 introns