No gene-specific primers found for LOC100331909. Showing all 10 primers including those that may amplify other genes. Consider validating with NCBI Primer-BLAST.

LOC100331909 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001365958 · chr22

118 3 genes · 3 transcripts

FWD 5' TGGACGGCAGCAGAATCATG 3'

REV 5' CAGAGTGCTCACACGCAGTT 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 132 bp

Spans 2 introns

NCBI BLAST

NM_001365958 · chr22

118 3 genes · 3 transcripts

FWD 5' GTGGACGGCAGCAGAATCAT 3'

REV 5' CAGAGTGCTCACACGCAGTT 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 133 bp

Spans 2 introns

NCBI BLAST

NM_001365958 · chr22

118 2 genes · 2 transcripts

FWD 5' CGGCAGCAGAATCATGCTCT 3'

REV 5' TCCAGGACAGAGTGCTCACA 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 135 bp

Spans 2 introns

NCBI BLAST

NM_001365958 · chr22

118 2 genes · 2 transcripts

FWD 5' CGGCAGCAGAATCATGCTCT 3'

REV 5' CAGAGTGCTCACACGCAGTT 3'

Fwd Tm 62.5°C

Rev Tm 62.4°C

GC 55%

Product 128 bp

Spans 2 introns

NCBI BLAST

NM_001365958 · chr22

118 4 genes · 4 transcripts

FWD 5' GTGGACGGCAGCAGAATCAT 3'

REV 5' TCCAGGACAGAGTGCTCACA 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 140 bp

Spans 2 introns

NCBI BLAST

NM_001365958 · chr22

118 4 genes · 4 transcripts

FWD 5' TGGACGGCAGCAGAATCATG 3'

REV 5' TCCAGGACAGAGTGCTCACA 3'

Fwd Tm 62.5°C

Rev Tm 62.5°C

GC 55%

Product 139 bp

Spans 2 introns

NCBI BLAST

NM_001365958 · chr22

117 4 genes · 4 transcripts

FWD 5' TTGTGGACGGCAGCAGAATC 3'

REV 5' TCCAGGACAGAGTGCTCACA 3'

Fwd Tm 62.6°C

Rev Tm 62.5°C

GC 55%

Product 142 bp

Spans 2 introns

NCBI BLAST

NM_001365958 · chr22

117 3 genes · 3 transcripts

FWD 5' TTGTGGACGGCAGCAGAATC 3'

REV 5' CAGAGTGCTCACACGCAGTT 3'

Fwd Tm 62.6°C

Rev Tm 62.4°C

GC 55%

Product 135 bp

Spans 2 introns

NCBI BLAST

NM_001365958 · chr22

117 2 genes · 2 transcripts

FWD 5' GACGGCAGCAGAATCATGCT 3'

REV 5' TCCAGGACAGAGTGCTCACA 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 137 bp

Spans 2 introns

NCBI BLAST

NM_001365958 · chr22

117 2 genes · 2 transcripts

FWD 5' ACGGCAGCAGAATCATGCTC 3'

REV 5' TCCAGGACAGAGTGCTCACA 3'

Fwd Tm 62.7°C

Rev Tm 62.5°C

GC 55%

Product 136 bp

Spans 2 introns

NCBI BLAST