LOC557301 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001327818 · chr7

110 Specific

FWD 5' ACCCGAATCTGACCAACGAA 3'

REV 5' ATCCTTTCCTGATGGCGCAC 3'

Fwd Tm 61.0°C

Rev Tm 62.5°C

GC 50%

Product 150 bp

Spans 2 introns

NM_001327818 · chr7

109 Specific

FWD 5' ACCCAAAGGACCCGAATCTG 3'

REV 5' TCGATCCTTTCCTGATGGCG 3'

Fwd Tm 61.7°C

Rev Tm 61.7°C

GC 55%

Product 162 bp

Spans 2 introns

NM_001327818 · chr7

109 Specific

FWD 5' GACCCGAATCTGACCAACGA 3'

REV 5' TCCTTTCCTGATGGCGCACT 3'

Fwd Tm 61.5°C

Rev Tm 63.8°C

GC 55%

Product 150 bp

Spans 2 introns

NM_001327818 · chr7

109 Specific

FWD 5' TGCTGGAGAAACTGCAGAGC 3'

REV 5' GATCCTTTCCTGATGGCGCA 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 103 bp

Spans 1 intron

NM_001327818 · chr7

109 Specific

FWD 5' GCTGGAGAAACTGCAGAGCA 3'

REV 5' GATCCTTTCCTGATGGCGCA 3'

Fwd Tm 62.4°C

Rev Tm 62.3°C

GC 55%

Product 102 bp

Spans 1 intron

NM_001327818 · chr7

108 Specific

FWD 5' CCTGCATACAGGCTGCAGAA 3'

REV 5' GCTCTGCAGTTTCTCCAGCA 3'

Fwd Tm 62.2°C

Rev Tm 62.4°C

GC 55%

Product 149 bp

Spans 1 intron

NM_001327818 · chr7

108 Specific

FWD 5' CCTGCATACAGGCTGCAGAA 3'

REV 5' TGCTCTGCAGTTTCTCCAGC 3'

Fwd Tm 62.2°C

Rev Tm 62.4°C

GC 55%

Product 150 bp

Spans 1 intron

NM_001327818 · chr7

108 Specific

FWD 5' GGACCCGAATCTGACCAACG 3'

REV 5' CCTTTCCTGATGGCGCACTG 3'

Fwd Tm 62.6°C

Rev Tm 63.5°C

GC 60%

Product 150 bp

Spans 2 introns

NM_001327818 · chr7

108 Specific

FWD 5' GACCCGAATCTGACCAACGA 3'

REV 5' TCGATCCTTTCCTGATGGCG 3'

Fwd Tm 61.5°C

Rev Tm 61.7°C

GC 55%

Product 154 bp

Spans 2 introns

NM_001327818 · chr7

108 Specific

FWD 5' GACCCGAATCTGACCAACGA 3'

REV 5' CCTTTCCTGATGGCGCACTG 3'

Fwd Tm 61.5°C

Rev Tm 63.5°C

GC 55%

Product 149 bp

Spans 2 introns