LOC558044 10 primer pairs found

Zebrafish

Show all (including non-specific)

NM_001099989 · chr5

117 Specific

FWD 5' GTCTGTGAGGTTGCCCATGA 3'

REV 5' GACTGGATGGCTCGAATCCA 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 131 bp

Spans 2 introns

NM_001099989 · chr5

117 Specific

FWD 5' TCTGTGAGGTTGCCCATGAC 3'

REV 5' GACTGGATGGCTCGAATCCA 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 130 bp

Spans 2 introns

NM_001099989 · chr5

117 Specific

FWD 5' CTGTCTGTGAGGTTGCCCAT 3'

REV 5' GACTGGATGGCTCGAATCCA 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 133 bp

Spans 2 introns

NM_001099989 · chr5

117 Specific

FWD 5' CTGTGAGGTTGCCCATGACT 3'

REV 5' GACTGGATGGCTCGAATCCA 3'

Fwd Tm 61.9°C

Rev Tm 61.4°C

GC 55%

Product 129 bp

Spans 2 introns

NM_001099989 · chr5

117 Specific

FWD 5' CTGTGAGGTTGCCCATGACT 3'

REV 5' CGACATGGAGGACTGGATGG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 139 bp

Spans 2 introns

NM_001099989 · chr5

117 Specific

FWD 5' GTCTGTGAGGTTGCCCATGA 3'

REV 5' CGACATGGAGGACTGGATGG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 141 bp

Spans 2 introns

NM_001099989 · chr5

117 Specific

FWD 5' TCTGTGAGGTTGCCCATGAC 3'

REV 5' CGACATGGAGGACTGGATGG 3'

Fwd Tm 61.9°C

Rev Tm 61.9°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001099989 · chr5

117 Specific

FWD 5' CTGTCTGTGAGGTTGCCCAT 3'

REV 5' CATGGAGGACTGGATGGCTC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 140 bp

Spans 2 introns

NM_001099989 · chr5

117 Specific

FWD 5' CTGTGAGGTTGCCCATGACT 3'

REV 5' CATGGAGGACTGGATGGCTC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 136 bp

Spans 2 introns

NM_001099989 · chr5

117 Specific

FWD 5' GTCTGTGAGGTTGCCCATGA 3'

REV 5' CATGGAGGACTGGATGGCTC 3'

Fwd Tm 61.9°C

Rev Tm 62.0°C

GC 55%

Product 138 bp

Spans 2 introns